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Product Info Summary
| SKU: | A10240-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PTCRA Antibody Picoband®
SKU/Catalog Number
A10240-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTCRA Antibody Picoband® catalog # A10240-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTCRA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10240-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PTCRA recombinant protein (Position: T24-T143).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10240-1 is reactive to PTCRA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
41409 MW
Background of Ptcra
This PTCRA gene is mapped to 6p21.1. The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10240-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A375 whole cell, human HL-60 whole cell, human CCRM-CEM whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTCRA using anti-PTCRA antibody (A10240-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysate,
Lane 2: human HL-60 whole cell lysate,
Lane 3: human CCRM-CEM whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCRA antigen affinity purified polyclonal antibody (Catalog # A10240-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTCRA at approximately 29KD. The expected band size for PTCRA is at 29KD.
Protein Target Info & Infographic
Gene/Protein Information For PTCRA (Source: Uniprot.org, NCBI)
Gene Name
PTCRA
Full Name
Pre T-cell antigen receptor alpha
Weight
41409 MW
Alternative Names
Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA Ptcra|gp33, pT, pT-, pT-alpha, pT-alpha-TCR, pT[, pT[a], pTa, pTalpha|pre T cell receptor alpha|pre T-cell receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTCRA, check out the PTCRA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTCRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTCRA Antibody Picoband® (A10240-1)
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Customer Q&As
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6 Customer Q&As for Anti-PTCRA Antibody Picoband®
Question
I was wanting to use to test anti-PTCRA antibody A10240-1 on human cingulate cortex for research purposes, then I may be interested in using anti-PTCRA antibody A10240-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The products we sell, including anti-PTCRA antibody A10240-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-11
Question
Do you have a BSA free version of anti-PTCRA antibody A10240-1 available?
T. Moore
Verified customer
Asked: 2020-02-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PTCRA antibody A10240-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-25
Question
Would A10240-1 anti-PTCRA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Moore
Verified customer
Asked: 2018-04-09
Answer
You can see on the product datasheet, A10240-1 anti-PTCRA antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-09
Question
Is a blocking peptide available for product anti-PTCRA antibody (A10240-1)?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
We do provide the blocking peptide for product anti-PTCRA antibody (A10240-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-01
Question
We are currently using anti-PTCRA antibody A10240-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
J. Moore
Verified customer
Asked: 2013-12-25
Answer
The anti-PTCRA antibody (A10240-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-25
Question
I was wanting to use your anti-PTCRA antibody for ELISA for human cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cingulate cortex identification?
J. Jha
Verified customer
Asked: 2013-03-27
Answer
You can see on the product datasheet, A10240-1 anti-PTCRA antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-27
