Anti-PTCRA Antibody Picoband™

Product Name

Anti-PTCRA Antibody Picoband™

View all Ptcra Antibodies

SKU/Catalog Number

A10240-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PTCRA Antibody Picoband™ catalog # A10240-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PTCRA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10240-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PTCRA recombinant protein (Position: T24-T143).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10240-1 is reactive to Ptcra in Human

Applications

A10240-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

21.491kDa

Background of Ptcra

This PTCRA gene is mapped to 6p21.1. The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PTCRA using anti-PTCRA antibody (A10240-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysate,
Lane 2: human HL-60 whole cell lysate,
Lane 3: human CCRM-CEM whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCRA antigen affinity purified polyclonal antibody (Catalog # A10240-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTCRA at approximately 29KD. The expected band size for PTCRA is at 29KD.

Gene/Protein Information For Ptcra (Source: Uniprot.org, NCBI)

Alternative Names

Pre T-cell antigen receptor alpha Ptcra|gp33, pT, pT-, pT-alpha, pT-alpha-TCR, pT[, pT[a], pTa, pTalpha|pre T cell antigen receptor alpha|pre T-cell antigen receptor alpha

For more info on Ptcra, check out the Ptcra Infographic

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In this infographic, you will see the following information for Ptcra: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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