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Product Info Summary
| SKU: | A15119 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-PTTG2 Antibody (Center)
SKU/Catalog Number
A15119
Size
80 µl
Description
Boster Bio Anti-PTTG2 Antibody (Center) (Catalog # A15119). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PTTG2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15119)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2.
Reactive Species
A15119 is reactive to PTTG2 in Human
Calculated molecular weight
22.3 kDa
Background of PTTG2
May be involved in transcriptional regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15119 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody (Center). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with PTTG2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PTTG2 immunoreactivity is localized to Cytoplasm significantly.
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Anti-PTTG2 Antibody (Center) at 1:2000 dilution + human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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PTTG2 Antibody (Center) (Cat. #A15119) western blot analysis in 293 cell line lysates (35ug/lane). This demonstrates the PTTG2 antibody detected the PTTG2 protein (arrow).
Specific Publications For Anti-PTTG2 Antibody (Center) (A15119)
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