Anti-Pentraxin-related protein PTX3 PTX3 Antibody

Product Name

Anti-Pentraxin-related protein PTX3 PTX3 Antibody

View all Pentraxin 3/TSG-14 Antibodies

SKU/Catalog Number

A00649

Size

50ug

Form

Liquid. In PBS containing 0.1% sodium azide.

Description

Boster Bio Anti-Pentraxin-related protein PTX3 PTX3 Antibody catalog # A00649. Tested in ELISA, IP, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pentraxin-related protein PTX3 PTX3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00649)

Host

Rabbit

Contents

Liquid. In PBS containing 0.1% sodium azide.

Clonality

Polyclonal

Clone Number

1559CT572.3.83

Isotype

IgG1,κ

Immunogen

Recombinant human PTX3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00649 is reactive to PTX3 in Human, Mouse, Rat

Applications

A00649 is guaranteed for ELISA, IP, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

41.976kDa

Background of Pentraxin 3/TSG-14

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (detection, 1:20,000)
Western Blot (500ng/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure:Lysates of normal lung fibroblasts (WI38) and of human fibrosarcoma (8387) were probed with the PTX3, pAb, (Prod. No. A00649), over night at +4 °C at a concentration of 500ng/ml in 5% dry milk in PBS+/+ containing0.05% Tween 20. The signal was revealed with an HRP-conjugated anti-rat IgG secondary antibody in 5% dry milk PBS+/+ containing 0.05% Tween 20 after incubation for 1 hour at room temperature and IMMOBILON Western Chemiluminescent substrate from Millipore.

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Figure 2. IHC analysis of PTX3 using anti-PTX3 antibody (A00649).
PTX3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PTX3 Antibody (A00649) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For PTX3 (Source: Uniprot.org, NCBI)

Alternative Names

alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14 PTX3 TNFAIP5, TSG-14 pentraxin 3 pentraxin-related protein PTX3|TNF alpha-induced protein 5|long pentraxin 3|tumor necrosis factor alpha-induced protein 5|tumor necrosis factor-inducible gene 14 protein|tumor necrosis factor-inducible protein TSG-14

For more info on PTX3, check out the PTX3 Infographic

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In this infographic, you will see the following information for PTX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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