Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody

Product Name

Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody

View all Pyruvate Dehydrogenase E1 beta subunit Antibodies

SKU/Catalog Number

M03998

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody catalog # M03998. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03998)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEAD-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03998 is reactive to PDHB in Human, Mouse, Rat

Applications

M03998 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

39.233kDa

Background of Pyruvate Dehydrogenase E1 beta subunit

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit expression in HeLa cell lysate.

Gene/Protein Information For PDHB (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp564K0164; mitochondrial; pyruvate dehydrogenase (lipoamide) beta; pyruvate dehydrogenase, E1 beta polypeptide PDHB PDHBD, PDHE1-B, PDHE1B, PHE1B pyruvate dehydrogenase E1 subunit beta pyruvate dehydrogenase E1 component subunit beta, mitochondrial|epididymis secretory sperm binding protein|pyruvate dehydrogenase (lipoamide) beta|pyruvate dehydrogenase E1 beta subunit|pyruvate dehydrogenase, E1 beta polypeptide

For more info on PDHB, check out the PDHB Infographic

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In this infographic, you will see the following information for PDHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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