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Product Info Summary
| SKU: | A02857-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-R-spondin-1 RSPO1 Antibody
SKU/Catalog Number
A02857-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-R-spondin-1 RSPO1 Antibody (Catalog # A02857-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
RSPO1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-R-spondin-1 RSPO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02857-1)
Host
Rabbit
Contents
RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-RSPO1 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.
Cross-reactivity
At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.
Reactive Species
A02857-1 is reactive to RSPO1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.0 kDa
Background of RSPO1
RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02857-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC: 2.5-5 μg/mL; IF: 5 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: RSPO1, A02857-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Spleen
Loading: 15 μg of lysates per lane.
Antibodies: RSPO1, A02857-1 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation with Recombinant Protein
Loading: 30 ng of human RSPO1 recombinant protein per lane.
Antibodies: RSPO1, A02857-1 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL, Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Heart
Loading: 15 μg of lysates per lane.
Antibodies: RSPO1, A02857-1 (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of RSPO1 in Human Spleen
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with A02857-1 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).
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Immunohistochemistry Validation of RSPO1 in Human Heart
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-RSPO1 antibody (A02857-1) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (A02857-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of RSPO1 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RSPO1 antibody (A02857-1) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-R-spondin-1 RSPO1 Antibody (A02857-1)
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