Product Info Summary
| SKU: | PA2282 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Ras-related protein Rab-11A Rab11A Antibody Picoband®
SKU/Catalog Number
PA2282
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ras-related protein Rab-11A Rab11A Antibody catalog # PA2282. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ras-related protein Rab-11A Rab11A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2282)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab11A, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2282 is reactive to RAB11A in Human, Mouse, Rat
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.4 kDa
Background of RAB11A
Ras-related protein Rab-11A is a protein that in humans is encoded by the RAB11A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family which plays essential roles in vesicle and granule targeting. It is mapped to 15q22.31. RAB11A is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. RAB11A also can control intracellular trafficking of the innate immune receptor TLR4, and thereby also receptor signaling. It has been shown to interact with RAB11FIP2, RAB11FIP4, RAB11FIP1 and so on.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2282 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
Positive Control
WB: HELA Whole Cell, NIH Whole Cell, A431 Whole Cell
IP: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Rab11A antibody, PA2282, All Western blotting
All lanes: Anti-RAB11A(PA2282) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: NIH Whole Cell Lysate at 40ug
Lane 3: A431 Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
Click image to see more details
Immunoprecipitating (IP) Rab11A in Hela whole cell lysate.
Western blot analysis of Rab11A using anti-Rab11A antibody (PA2282);
Lane 1: Hela whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-Rab11A antibody in Hela whole cell lysate;
Lane 3: anti-Rab11A antibody (2μg) + Hela whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (PA2282) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa.
Specific Publications For Anti-Ras-related protein Rab-11A Rab11A Antibody Picoband® (PA2282)
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Customer Q&As
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7 Customer Q&As for Anti-Ras-related protein Rab-11A Rab11A Antibody Picoband®
Question
I see that the anti-Rab11A antibody PA2282 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-22
Question
Do you have a BSA free version of anti-Rab11A antibody PA2282 available?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab11A antibody PA2282 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-03
Question
I was wanting to use your anti-Rab11A antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-03-14
Answer
It shows on the product datasheet, PA2282 anti-Rab11A antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-14
Question
Is a blocking peptide available for product anti-Rab11A antibody (PA2282)?
K. Zhao
Verified customer
Asked: 2017-05-08
Answer
We do provide the blocking peptide for product anti-Rab11A antibody (PA2282). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-05-08
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Rab11A antibody PA2282. Let me know if you need anything else.
J. Miller
Verified customer
Asked: 2017-03-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-30
Question
Is this PA2282 anti-Rab11A antibody reactive to the isotypes of RAB11A?
K. Johnson
Verified customer
Asked: 2014-05-13
Answer
The immunogen of PA2282 anti-Rab11A antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Rab11A(194-211aa NVVPIHVPPTTENKPKVQ), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-05-13
Question
Does anti-Rab11A antibody PA2282 work for WB with cervix carcinoma?
A. Singh
Verified customer
Asked: 2014-04-04
Answer
According to the expression profile of cervix carcinoma, RAB11A is highly expressed in cervix carcinoma. So, it is likely that anti-Rab11A antibody PA2282 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2014-04-04


