Product Info Summary
SKU: | PB9431 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Rac1 Antibody Picoband™
SKU/Catalog Number
PB9431
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rac1 Antibody Picoband™ catalog # PB9431. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rac1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9431)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rac1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9431 is reactive to RAC1 in Human, Mouse, Rat
Applications
PB9431 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
21 kDa
Calculated molecular weight
21.45kDa
Background of Rac1
Rac1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1gene. This gene can produce a variety of alternatively splicedversions of the Rac1 protein, which appear to carry out different functions. This gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RAC1 using anti-RAC1 antibody (PB9431).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Pancreas Tissue Lysate at 50ug,
Lane 2: Mouse Intestine Tissue Lysate at 50ug,
Lane 3: HELA Whole Cell Lysate at 40ug,
Lane 4: COLO320 Whole Cell Lysate at 40ug,
Lane 5: SGC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAC1 antigen affinity purified polyclonal antibody (Catalog # PB9431) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAC1 at approximately 21 kDa. The expected band size for RAC1 is at 21 kDa.
Click image to see more details
Figure 2. IF analysis of Rac1 using anti-Rac1 antibody (PB9431).
Rac1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Rac1 Antibody (PB9431) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For RAC1 (Source: Uniprot.org, NCBI)
Gene Name
RAC1
Full Name
Ras-related C3 botulinum toxin substrate 1
Weight
21.45kDa
Superfamily
small GTPase superfamily
Alternative Names
Cell migration-inducing gene 5 protein; MGC111543; p21-Rac1; p21-Rac1migration-inducing gene 5; Rac1; Ras-like protein TC25; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP bindingprotein Rac1); ras-related C3 botulinum toxin substrate 1; rho family, small GTP binding protein Rac1; TC25; TC-25 RAC1 MIG5, MRD48, Rac-1, TC-25, p21-Rac1 Rac family small GTPase 1 ras-related C3 botulinum toxin substrate 1|cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAC1, check out the RAC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rac1 Antibody Picoband™ (PB9431)
Hello CJ!
PB9431 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Han F1, Zhuang TT1, Chen JJ1, Zhu XL1,2, Cai YF1, Lu YP1. PLoS One. 2017 Sep 21;12(9):e0185102. doi: 10.1371/journal.pone.0185102. eCollection 2017. Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal ...
Shen L, Yang A, Yao P, Sun X, Chen C, Mo C, Shi L, Chen Y, Liu Q. Biometals. 2014 Aug;27(4):753-62. Doi: 10.1007/S10534-014-9769-9. Epub 2014 Jul 19. Gadolinium Promoted Proliferation In Mouse Embryo Fibroblast Nih3T3 Cells Through Rac And Pi3K/Ak...
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
17 Customer Q&As for Anti-Rac1 Antibody Picoband™
Question
Do you have a BSA free version of anti-Rac1 antibody PB9431 available?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Rac1 antibody PB9431 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-17
Question
Does anti-Rac1 antibody PB9431 work for WB with colon skin?
A. Lewis
Verified customer
Asked: 2020-01-15
Answer
According to the expression profile of colon skin, RAC1 is highly expressed in colon skin. So, it is likely that anti-Rac1 antibody PB9431 will work for WB with colon skin.
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use your anti-Rac1 antibody for WB for mouse colon skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon skin identification?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can see on the product datasheet, PB9431 anti-Rac1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
Does anti-Rac1 antibody PB9431 work on goat WB with esophagus squamous epithelium?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Our lab technicians have not tested anti-Rac1 antibody PB9431 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Rac1 antibody PB9431 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat esophagus squamous epithelium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-26
Question
Is this PB9431 anti-Rac1 antibody reactive to the isotypes of RAC1?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The immunogen of PB9431 anti-Rac1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Rac1 (169-189aa FDEAIRAVLCPPPVKKRKRKC), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-06
Question
Does PB9431 crossreact with Rac2?
Verified customer
Asked: 2019-10-29
Answer
For the Anti-Rac1 Antibody Picoband™ (PB9431), our lab hasn't experimentally validated cross-reactivity with Rac2. Currently we haven't got a Rac1 antibody that does not cross-react with Rac2.
Boster Scientific Support
Answered: 2019-10-30
Question
you antibody using your anti-Rac1 antibody for activation of rac1 studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Thank you for your inquiry. This PB9431 anti-Rac1 antibody is validated on rat pancreas tissue, tissue lysate, mouse intestine tissue, hela whole cell lysate, colo320 whole cell lysate, sgc whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-28
Question
My team were satisfied with the WB result of your anti-Rac1 antibody. However we have been able to see positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is RAC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
According to literature, cervix carcinoma does express RAC1. Generally RAC1 expresses in cell membrane. Regarding which tissues have RAC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Skin, Pubmed ID: 10597294
Melanoma, Pubmed ID: 17081065
Pancreas, and Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-10
Question
I see that the anti-Rac1 antibody PB9431 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-23
Question
I have attached the WB image, lot number and protocol we used for colon skin using anti-Rac1 antibody PB9431. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon skin using anti-Rac1 antibody PB9431. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-19
Question
We are currently using anti-Rac1 antibody PB9431 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
R. Kulkarni
Verified customer
Asked: 2019-03-11
Answer
The anti-Rac1 antibody (PB9431) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-11
Question
We have observed staining in rat colon skin. Any tips? Is anti-Rac1 antibody supposed to stain colon skin positively?
A. Williams
Verified customer
Asked: 2018-09-25
Answer
Based on literature colon skin does express RAC1. Based on Uniprot.org, RAC1 is expressed in esophagus squamous epithelium, colon skin, pancreas skin, melanoma, cervix carcinoma, among other tissues. Regarding which tissues have RAC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Skin, Pubmed ID: 10597294
Melanoma, Pubmed ID: 17081065
Pancreas, and Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-09-25
Question
Is a blocking peptide available for product anti-Rac1 antibody (PB9431)?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
We do provide the blocking peptide for product anti-Rac1 antibody (PB9431). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-20
Question
My question regarding product PB9431, anti-Rac1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Krishna
Verified customer
Asked: 2015-06-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9431 anti-Rac1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-29
Question
I would like to test anti-Rac1 antibody PB9431 on mouse colon skin for research purposes, then I may be interested in using anti-Rac1 antibody PB9431 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Miller
Verified customer
Asked: 2015-03-09
Answer
The products we sell, including anti-Rac1 antibody PB9431, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-03-09
Question
Does PB9431 anti-Rac1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Banerjee
Verified customer
Asked: 2014-01-14
Answer
It shows on the product datasheet, PB9431 anti-Rac1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-01-14