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Product Info Summary
| SKU: | M01714-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RAC2 Antibody (Monoclonal, 31R93)
SKU/Catalog Number
M01714-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-RAC2 Antibody (Monoclonal, 31R93) catalog # M01714-1. Tested in WB, IHC, IF, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAC2 Antibody (Monoclonal, 31R93) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01714-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31R93
Immunogen
Recombinant full length protein of Human RAC1 aa 3-190.
Reactive Species
M01714-1 is reactive to RAC2 in Human, Mouse, Rat
Observed Molecular Weight
21 kDa
Calculated molecular weight
21.4 kDa
Background of RAC2
This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01714-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunofluorescence, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Positive Control
WB: human THP-1 whole cell lysates, human HEL whole cell lysates, human Jurkat whole cell lysates, mouse spleen tissue lysates, mouse thymus tissue lysates
Validation Images & Assay Conditions
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Western blot analysis of RAC2 using anti-RAC2 antibody (M01714-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAC2 antigen affinity purified monoclonal antibody (M01714-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAC2 at approximately 21 kDa. The expected band size for RAC2 is at 21 kDa.
Specific Publications For Anti-RAC2 Antibody (Monoclonal, 31R93) (M01714-1)
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