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Product Info Summary
| SKU: | A20019 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RAD51L1 RAD51B Antibody
SKU/Catalog Number
A20019
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RAD51L1 RAD51B Antibody (Catalog# A20019). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAD51L1 RAD51B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A20019)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RAD51L1. Immunogen sequence location: 201-250
Reactive Species
A20019 is reactive to RAD51B in Human, Monkey, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
42.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A20019 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using RAD51L1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COS7 cells, using RAD51L1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RAD51L1 RAD51B Antibody (A20019)
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Customer Q&As
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4 Customer Q&As for Anti-RAD51L1 RAD51B Antibody
Question
We are currently using anti-RAD51L1 antibody A20019 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
The anti-RAD51L1 antibody (A20019) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-22
Question
Is there a BSA free version of anti-RAD51L1 antibody A20019 available?
A. Gonzalez
Verified customer
Asked: 2017-07-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RAD51L1 antibody A20019 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-10
Question
I see that the anti-RAD51L1 antibody A20019 works with WB, what is the protocol used to produce the result images on the product page?
K. Jackson
Verified customer
Asked: 2017-06-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-06-01
Question
Will anti-RAD51L1 antibody A20019 work on feline IHC with corpus callosum?
G. Zhang
Verified customer
Asked: 2014-07-08
Answer
Our lab technicians have not validated anti-RAD51L1 antibody A20019 on feline. You can run a BLAST between feline and the immunogen sequence of anti-RAD51L1 antibody A20019 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline corpus callosum in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-08
