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Product Info Summary
| SKU: | A06883-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Rag A/B RRAGA Antibody
SKU/Catalog Number
A06883-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rag A/B RRAGA Antibody catalog # A06883-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rag A/B RRAGA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06883-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Rag A/B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06883-1 is reactive to RRAGA in Human, Mouse, Rat
Applications
A06883-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.566kDa
Background of RRAGA
Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
Ota T., Nat. Genet. 36:40-45(2004).
Scherer S.E., Nature 440:346-351(2006).
Channavajhala P.L., J. Biol. Chem. 278:47089-47097(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
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Figure 3. Immunohistochemistry validation of RRAGA using Anti-Rag A/B RRAGA Antibody (A06883-1).
Immunohistochemical analysis of paraffin-embedded Human brain using Anti-Rag A/B polyclonal antibody. Antibody was diluted at 1:100 (4°C
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Figure 4. Immunohistochemistry validation of RRAGA using Anti-Rag A/B RRAGA Antibody (A06883-1).
Immunohistochemical analysis of paraffin-embedded Human brain using Anti-Rag A/B polyclonal antibody. Antibody was diluted at 1:100 (4°C
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Figure 1. Western blotting validation for Anti-Rag A/B RRAGA Antibody A06883-1
Western Blot (WB) analysis of mouse brain cells using Anti-Rag A/B polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Figure 2. Western blotting validation for Anti-Rag A/B RRAGA Antibody A06883-1
Western Blot (WB) analysis of HepG2 cells using Anti-Rag A/B polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For RRAGA (Source: Uniprot.org, NCBI)
Gene Name
RRAGA
Full Name
Ras-related GTP-binding protein A
Weight
36.566kDa
Superfamily
GTR/RAG GTP-binding protein family
Alternative Names
Ras-related GTP-binding protein A RRAGA FIP-1, FIP1, RAGA Ras related GTP binding A ras-related GTP-binding protein A|adenovirus E3 14.7 kDa-interacting protein 1|adenovirus E3-14.7K interacting protein 1|rag A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRAGA, check out the RRAGA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRAGA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rag A/B RRAGA Antibody (A06883-1)
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No publications found for A06883-1
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