Product Info Summary
SKU: | A03547 |
---|---|
Size: | 100 µg |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-RAP2A Antibody
SKU/Catalog Number
A03547
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-RAP2A Antibody (Catalog# A03547). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-RAP2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03547)
Host
Rabbit
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Full length recombinant RAP2A protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03547 is reactive to RAP2A in Human
Applications
A03547 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
20.615kDa
Background of RAP2A
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 1-2 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of RAP2A. Anti-RAP2A antibody was used at 1 &mu
Protein Target Info & Infographic
Gene/Protein Information For RAP2A (Source: Uniprot.org, NCBI)
Gene Name
RAP2A
Full Name
Ras-related protein Rap-2a
Weight
20.615kDa
Superfamily
small GTPase superfamily
Alternative Names
KREV; K-REV; RAP2; RAP2, member of RAS oncogene family (K-rev); Rap2A; RAP2A, member of RAS oncogene family; ras-related protein Rap-2a; RbBP-30 RAP2A K-REV, KREV, RAP2, RbBP-30 RAP2A, member of RAS oncogene family ras-related protein Rap-2a|RAP2, member of RAS oncogene family (K-rev)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAP2A, check out the RAP2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAP2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAP2A Antibody (A03547)
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No publications found for A03547
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-RAP2A Antibody
Question
Does anti-RAP2A antibody A03547 work on dog WB with lymph node?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Our lab technicians have not tested anti-RAP2A antibody A03547 on dog. You can run a BLAST between dog and the immunogen sequence of anti-RAP2A antibody A03547 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog lymph node in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
Do you have a BSA free version of anti-RAP2A antibody A03547 available?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-RAP2A antibody A03547 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-21
Question
We are currently using anti-RAP2A antibody A03547 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
The anti-RAP2A antibody (A03547) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-07
Question
Would A03547 anti-RAP2A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Carter
Verified customer
Asked: 2017-06-20
Answer
As indicated on the product datasheet, A03547 anti-RAP2A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-20
Question
Is a blocking peptide available for product anti-RAP2A antibody (A03547)?
R. Li
Verified customer
Asked: 2015-06-25
Answer
We do provide the blocking peptide for product anti-RAP2A antibody (A03547). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-06-25
Question
Does anti-RAP2A antibody A03547 work for WB with lymph node?
E. Carter
Verified customer
Asked: 2013-06-12
Answer
According to the expression profile of lymph node, RAP2A is highly expressed in lymph node. So, it is likely that anti-RAP2A antibody A03547 will work for WB with lymph node.
Boster Scientific Support
Answered: 2013-06-12