Anti-RBPJK/RBPJ Picoband™ Antibody (monoclonal, 6G4)

Product Name

Anti-RBPJK/RBPJ Picoband™ Antibody (monoclonal, 6G4)

View all csl/RBPJK Antibodies

SKU/Catalog Number

M00767-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RBPJK/RBPJ Picoband™ Antibody (monoclonal, 6G4) catalog # M00767-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RBPJK/RBPJ Picoband™ Antibody (monoclonal, 6G4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00767-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Monoclonal

Clone Number

6G4

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human RBPJK/RBPJ recombinant protein (Position: K41-Q467).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00767-1 is reactive to RBPJ in Human, Mouse, Rat

Applications

M00767-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

55.637kDa

Background of csl/RBPJK

Recombination signal binding protein for immunoglobulin kappa J region is a protein that in humans is encoded by the RBPJ gene. It is mapped to 4p15.2. The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RBPJK/RBPJ using anti-RBPJK/RBPJ antibody (M00767-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: human HELA whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: human HL-60 whole cell lysates,
Lane 7: rat NRK whole cell lysates,
Lane 8: mouse smooth muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RBPJK/RBPJ antigen affinity purified monoclonal antibody (Catalog # M00767-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RBPJK/RBPJ at approximately 56KD. The expected band size for RBPJK/RBPJ is at 56KD.

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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-RBPJK/RBPJ antibody (M00767-1).
Overlay histogram showing HEPA1-6 cells stained with M00767-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RBPJK/RBPJ Antibody (M00767-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of U937 cells using anti-RBPJK/RBPJ antibody (M00767-1).
Overlay histogram showing U937 cells stained with M00767-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RBPJK/RBPJ Antibody (M00767-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RBPJ (Source: Uniprot.org, NCBI)

Alternative Names

CBF1; CBF-1; CSL; H-2K binding factor-2; IGKJRB1; IGKJRB1RBPSUHCBF1; IGKJRBRBP-J kappa; immunoglobulin kappa J region recombination signal binding protein 1; J kappa-recombination signal-binding protein; KBF2; RBPJ; RBP-JK; RBPJKMGC61669; RBP-Jrecombining binding protein suppressor of hairless (Drosophila); RBPSUH; recombination signal binding protein for immunoglobulin kappa J region; recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; SUH; suppressor of hairless homolog RBPJ AOS3, CBF-1, CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBP-J kappa, RBP-JKK, RBPSUH, SUH, csl, RBPJ recombination signal binding protein for immunoglobulin kappa J region recombining binding protein suppressor of hairless|H-2K binding factor-2|immunoglobulin kappa J region recombination signal binding protein 1|renal carcinoma antigen NY-REN-30|suppressor of hairless homolog

For more info on RBPJ, check out the RBPJ Infographic

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In this infographic, you will see the following information for RBPJ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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