Click image to see more details
Product Info Summary
| SKU: | A30447 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RFA2 Antibody
SKU/Catalog Number
A30447
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RFA2 Antibody (Catalog# A30447). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RFA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30447)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RFA2. Immunogen sequence location: 10-59
Reactive Species
A30447 is reactive to RPA2 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30447 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from K562 cells, using RFA2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RFA2 Antibody (A30447)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-RFA2 Antibody
Question
Is a blocking peptide available for product anti-RFA2 antibody (A30447)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We do provide the blocking peptide for product anti-RFA2 antibody (A30447). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-28
Question
I was wanting to use to test anti-RFA2 antibody A30447 on human cervix carcinoma for research purposes, then I may be interested in using anti-RFA2 antibody A30447 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Parker
Verified customer
Asked: 2019-01-21
Answer
The products we sell, including anti-RFA2 antibody A30447, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-21
Question
I see that the anti-RFA2 antibody A30447 works with IF, what is the protocol used to produce the result images on the product page?
Z. Parker
Verified customer
Asked: 2014-04-08
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-04-08
Question
Is this A30447 anti-RFA2 antibody reactive to the isotypes of RPA2?
M. Jha
Verified customer
Asked: 2014-01-07
Answer
The immunogen of A30447 anti-RFA2 antibody is A peptide derived from human RFA2. Immunogen sequence location: 10-59. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-07
