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Product Info Summary
| SKU: | A17313 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RGS21 Antibody
SKU/Catalog Number
A17313
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RGS21 Antibody (Catalog # A17313). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
RGS21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RGS21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17313)
Host
Rabbit
Contents
RGS21 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
RGS21 antibody was raised against a 16 amino acid synthetic peptide near the center of human RGS21. The immunogen is located within amino acids 40 - 90 of RGS21.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This MettL7B antibody is predicted to not cross-react with MettL7A.
Reactive Species
A17313 is reactive to RGS21 in Human
Applications
A17313 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
17.671kDa
Background of RGS21
Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RGS21 antibody can be used for detection of RGS21 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RGS21 in HepG2 cell lysate with RGS21 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of RGS21 in HepG2 cells with RGS21 antibody at 2.5 μg/mL.
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Immunofluorescence of RGS21 in HepG2 cells with RGS21 antibody at 20 μg/mL.
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Immunofluorescence of RGS21 in HepG2 cells with RGS21 antibody at 20 μg/mL.
Green: RGS21 Antibody (A17313)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For RGS21 (Source: Uniprot.org, NCBI)
Gene Name
RGS21
Full Name
Regulator of G-protein signaling 21
Weight
17.671kDa
Alternative Names
Regulator of G-protein signaling 21 RGS21 regulator of G protein signaling 21 regulator of G-protein signaling 21|regulator of G-protein signalling 21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RGS21, check out the RGS21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RGS21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RGS21 Antibody (A17313)
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No publications found for A17313
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Customer Reviews
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