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Product Info Summary
| SKU: | A10214 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-RHOBTB3 Antibody
SKU/Catalog Number
A10214
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RHOBTB3 Antibody catalog # A10214. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RHOBTB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10214)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RHOBTB3. AA range:335-384
Reactive Species
A10214 is reactive to RHOBTB3 in Human, Mouse
Calculated molecular weight
69.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10214 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC:1:50-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using RHOBTB3 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of customer's lysis using RHOBTB3 antibody. Antibody was diluted at 1:2000
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RHOBTB3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RHOBTB3 Antibody (A10214)
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Customer Q&As
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7 Customer Q&As for Anti-RHOBTB3 Antibody
Question
Is a blocking peptide available for product anti-RHOBTB3 antibody (A10214)?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We do provide the blocking peptide for product anti-RHOBTB3 antibody (A10214). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-13
Question
I have attached the WB image, lot number and protocol we used for chorionic villus using anti-RHOBTB3 antibody A10214. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-RHOBTB3 antibody A10214 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
D. Taylor
Verified customer
Asked: 2019-12-18
Answer
The anti-RHOBTB3 antibody (A10214) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
Question
I see that the anti-RHOBTB3 antibody A10214 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-24
Question
Does A10214 anti-RHOBTB3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Miller
Verified customer
Asked: 2019-09-16
Answer
As indicated on the product datasheet, A10214 anti-RHOBTB3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for chorionic villus using anti-RHOBTB3 antibody A10214. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-28
Question
Does anti-RHOBTB3 antibody A10214 work on zebrafish WB with hippocampus?
T. Parker
Verified customer
Asked: 2013-01-08
Answer
Our lab technicians have not validated anti-RHOBTB3 antibody A10214 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-RHOBTB3 antibody A10214 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish hippocampus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-08
