Anti-RIG-1 DDX58 Antibody

Product Name

Anti-RIG-1 DDX58 Antibody

View all RIG-I Antibodies

SKU/Catalog Number

A00244

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RIG-1 DDX58 Antibody (Catalog # A00244). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RIG-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RIG-1 DDX58 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00244)

Host

Rabbit

Contents

RIG-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RIG-1 antibody was raised against human GST-tagged RIG-1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.

Reactive Species

A00244 is reactive to DDX58 in Human, Mouse, Rat

Applications

A00244 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

106.6kDa

Background of RIG-I

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKε through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RIG-1 antibody can be used for the detection of RIG-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of RIG-1 in Lane 1) A20 cell lysate and Lane 2) Rat heart tissue lysate with RIG-1 antibody at 2 μg/mL.

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Immunohistochemistry of RIG-1 in mouse testis tissue with RIG-1 Antibody at 1 μg/mL.

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Immunohistochemistry of RIG-1 in rat heart tissue with RIG-1 Antibody at 10 μg/mL.

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Immunofluorescence of RIG-1 in human testis tissue with RIG-1 Antibody at 20 μg/mL.

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Immunofluorescence of RIG-1 in rat testis tissue with RIG-1 Antibody at 20 μg/mL.

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Immunofluorescence of RIG-1 in mouse testis tissue with RIG-1 Antibody at 20 μg/mL.

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Immunofluorescence of RIG-1 in MCF7 cells with RIG-1 Antibody at 20 μg/mL.

Gene/Protein Information For DDX58 (Source: Uniprot.org, NCBI)

Alternative Names

DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DKFZp686N19181; EC 3.6.4.13; FLJ13599; probable ATP-dependent RNA helicase DDX58; retinoic acid inducible gene I; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; RIG-1; RIGI; RIG-I; RIG-IDKFZp434J1111; RNA helicase RIG-I DDX58 RIG-I, RIG1, RIGI, RLR-1, SGMRT2 DExD/H-box helicase 58 antiviral innate immune response receptor RIG-I|DEAD (Asp-Glu-Ala-Asp) box polypeptide 58|DEAD box protein 58|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RNA helicase RIG-I|probable ATP-dependent RNA helicase DDX58|retinoic acid-inducible gene 1 protein|retinoic acid-inducible gene I protein

For more info on DDX58, check out the DDX58 Infographic

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In this infographic, you will see the following information for DDX58: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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