Click image to see more details
Product Info Summary
| SKU: | PA2242 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RIP3/RIPK3 Antibody
View all RIPK3/RIP3 Antibodies
SKU/Catalog Number
PA2242
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIP3/RIPK3 Antibody catalog # PA2242. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIP3/RIPK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2242)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RIP3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2242 is reactive to RIPK3 in Human
Applications
PA2242 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
56 kDa, 77 kDa
Calculated molecular weight
56.887kDa
Background of RIPK3/RIP3
Receptor-interacting serine/threonine-protein kinase 3 (RIPK3), also known as RIP3 is an enzyme that in humans is encoded by the RIPK3 gene. This gene is mapped to 14q12. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-RIP3 antibody, PA2242, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: SKOV-3 Cell Lysate
Lane 4: M231 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For RIPK3 (Source: Uniprot.org, NCBI)
Gene Name
RIPK3
Full Name
Receptor-interacting serine/threonine-protein kinase 3
Weight
56.887kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11.1; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine-threonine kinase 3; RIP3; RIP-3; RIP3receptor interacting protein 3; RIPK3; RIP-like protein kinase 3 RIPK3 RIP3 receptor interacting serine/threonine kinase 3 receptor-interacting serine/threonine-protein kinase 3|RIP-3|RIP-like protein kinase 3|receptor interacting protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIPK3, check out the RIPK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIP3/RIPK3 Antibody (PA2242)
Hello CJ!
PA2242 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Smad3-Targeted Therapy Protects against Cisplatin-Induced AKI by Attenuating Programmed Cell Death and Inflammation via a NOX4-Dependent Mechanism. Qin Yang,Li Gao,Xiao-wei Hu,Jia-nan Wang,Yao Zhang,Yu-hang Dong,Hui Yao Lan,Xiao-ming Meng
Species: Mouse
PA2242 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RIP3/RIPK3 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-RIP3/RIPK3 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-RIP3/RIPK3 Antibody
Question
We are currently using anti-RIP3/RIPK3 antibody PA2242 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-RIP3/RIPK3 antibody (PA2242) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Our team were happy with the WB result of your anti-RIP3/RIPK3 antibody. However we have observed positive staining in aortic endothelium fetal brain cytosol using this antibody. Is that expected? Could you tell me where is RIPK3 supposed to be expressed?
L. Miller
Verified customer
Asked: 2019-08-05
Answer
Based on literature, aortic endothelium fetal brain does express RIPK3. Generally RIPK3 expresses in cytoplasm, cytosol. Regarding which tissues have RIPK3 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, and Fetal brain, Pubmed ID: 10358032
Blood, Pubmed ID: 15489334
Cervix carcinoma, and Lymphocyte, Pubmed ID: 10339433
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-05
Question
We have been able to see staining in human blood. Any tips? Is anti-RIP3/RIPK3 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
According to literature blood does express RIPK3. According to Uniprot.org, RIPK3 is expressed in blood, cervix carcinoma lymphocyte, aortic endothelium fetal brain, thalamus, among other tissues. Regarding which tissues have RIPK3 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, and Fetal brain, Pubmed ID: 10358032
Blood, Pubmed ID: 15489334
Cervix carcinoma, and Lymphocyte, Pubmed ID: 10339433
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-09
Question
We need using your anti-RIP3/RIPK3 antibody for thymus development studies. Has this antibody been tested with western blotting on sw620 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
Thanks for your inquiry. This PA2242 anti-RIP3/RIPK3 antibody is validated on panc cell lysate, sw620 cell lysate, m231 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-25
