Anti-Rkhd4 MEX3A Antibody

Product Name

Anti-Rkhd4 MEX3A Antibody

View all MEX3A Antibodies

SKU/Catalog Number

A15239

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Rkhd4 MEX3A Antibody (Catalog # A15239). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Rkhd4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Rkhd4 MEX3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15239)

Host

Rabbit

Contents

Rkhd4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rkhd4 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Rkhd4. The immunogen is located within amino acids 420 - 470 of Rkhd4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.

Reactive Species

A15239 is reactive to MEX3A in Human, Mouse, Rat

Applications

A15239 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

54.173kDa

Background of MEX3A

Rkhd4, also known as MEX3A is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd4, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. While Rkhd2 has been suggested to be associated with susceptibility to essential hypertension type 8, the function of Rkhd4 remains unknown. Rkhd3 and Rkhd4, but not Rkhd2, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Rkhd4 antibody can be used for detection of Rkhd4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Rkhd4 in SK-N-SH cell lysate with Rkhd4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of Rkhd4 in human brain tissue with Rkhd4 antibody at 2.5 μg/mL.

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Immunofluorescence of rkhd4 in human brain tissue with rkhd4 antibody at 20 μg/mL.

Gene/Protein Information For MEX3A (Source: Uniprot.org, NCBI)

Alternative Names

MEX3A mex-3 homolog A; MEX-3A; ring finger and KH domain containing 4; RING finger and KH domain-containing protein 4; RKHD4; RNA-binding protein MEX3A MEX3A MEX-3A, RKHD4, RNF162 mex-3 RNA binding family member A RNA-binding protein MEX3A|RING finger and KH domain-containing protein 4|ring finger and KH domain containing 4|ring finger and KH domain containing protein

For more info on MEX3A, check out the MEX3A Infographic

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In this infographic, you will see the following information for MEX3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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