Product Info Summary
SKU: | A30664 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-RNF125 Antibody
SKU/Catalog Number
A30664
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RNF125 Antibody (Catalog# A30664). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF125 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30664)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RNF125. Immunogen sequence location: 131-180
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30664 is reactive to RNF125 in Human, Mouse, Rat
Applications
A30664 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
26.454kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from K562 and HT-29 cells, using RNF125 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RNF125 (Source: Uniprot.org, NCBI)
Gene Name
RNF125
Full Name
E3 ubiquitin-protein ligase RNF125
Weight
26.454kDa
Alternative Names
E3 ubiquitin-protein ligase RNF125; EC 6.3.2.-; FLJ20456; MGC21737; ring finger protein 125TRAC1; T-cell RING activation protein 1; T-cell ring protein identified in activation screen; TRAC-1 RNF125 TNORS, TRAC-1, TRAC1 ring finger protein 125 E3 ubiquitin-protein ligase RNF125|T-cell RING activation protein 1|T-cell ring protein identified in activation screen|ring finger protein 125, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF125, check out the RNF125 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF125: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF125 Antibody (A30664)
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No publications found for A30664
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-RNF125 Antibody
Question
We are currently using anti-RNF125 antibody A30664 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
The anti-RNF125 antibody (A30664) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-21