Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody

Boster Bio Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody catalog # A02236-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: A02236-Dyl550
Size: 50ug/vial
Reactive Species: Mouse
Host: Rabbit
Application: Flow Cytometry

Product Name

Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody

See all CD8 products

SKU/Catalog Number

A02236-Dyl550

Size

50ug/vial

Form

Liquid

Description

Boster Bio Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody catalog # A02236-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Mouse.

Storage & Handling

At 2-8?C for one year from date of receipt. Protect from light. Do not freeze.

Cite This Product

Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A02236-Dyl550 is reactive to Cd8a in Mouse

Applications

A02236-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For Cd8a (Source: Uniprot.Org, NCBI)

Uniprot ID

P01731

Gene ID

12525

Gene Name

Cd8a

Full Name

T-cell surface glycoprotein CD8 alpha chain

Weight

27.456kDa

Alternative Names

53-6.7 CD8 Alpha; 53-6.7 CD8 Aplha; 53-6.7 CD8; 53-6.7 Clone; 53-6.7; CD8; CD8; CD8a molecule; CD8A; Cytotoxic T cell marker; Cytotoxic T lymphocyte marker; LEU2; p32; T cell marker Cd8a|BB154331, Ly-, Ly-2, Ly-3, Ly-35, Ly-B, Lyt-, Lyt-2|CD8 antigen, alpha chain|T-cell surface glycoprotein CD8 alpha chain|Lyt-2.1 lymphocyte differentiation antigen (AA at 100)|T-cell surface glycoprotein Lyt-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd8a, check out the Cd8a Infographic

Cd8a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd8a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A02236-Dyl550 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Myeloid differentiation factor 88 promotes cisplatin chemoresistance in ovarian cancer

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14 Customer Q&As for Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody

Question

Is this A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody reactive to the isotypes of CD8A?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

The immunogen of A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody is E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-05-01

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-19

Question

Will anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 work for Flow Cytometry with thymus?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

According to the expression profile of thymus, CD8A is highly expressed in thymus. So, it is likely that anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 will work for Flow Cytometry with thymus.

Boster Scientific Support

Answered: 2019-08-29

Question

Does A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

You can see on the product datasheet, A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-24

Question

Is there a BSA free version of anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 available?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-12

Question

We have observed staining in mouse thymus. Are there any suggestions? Is anti-Mouse CD8 alpha DyLight® 550 conjugated antibody supposed to stain thymus positively?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

From what I have seen in literature thymus does express CD8A. From what I have seen in Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-07-10

Question

My boss were well pleased with the WB result of your anti-Mouse CD8 alpha DyLight® 550 conjugated antibody. However we have observed positive staining in pericardium isoform 2: secreted using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

From what I have seen in literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-05-29

Question

I was wanting to use your anti-Mouse CD8 alpha DyLight® 550 conjugated antibody for Flow Cytometry for mouse thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thymus identification?

Verified Customer

Verified customer

Asked: 2018-06-11

Answer

It shows on the product datasheet, A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody has been validated for Flow Cytometry on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse thymus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-11

Question

I see that the anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-26

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-26

Question

Is a blocking peptide available for product anti-Mouse CD8 alpha DyLight® 550 conjugated antibody (A02236-Dyl550)?

Verified Customer

Verified customer

Asked: 2017-10-18

Answer

We do provide the blocking peptide for product anti-Mouse CD8 alpha DyLight® 550 conjugated antibody (A02236-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-18

Question

We are currently using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on feline tissues as well?

N. Jha

Verified customer

Asked: 2017-10-11

Answer

The anti-Mouse CD8 alpha DyLight® 550 conjugated antibody (A02236-Dyl550) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-11

Question

We are interested in to test anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 on mouse thymus for research purposes, then I may be interested in using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Carter

Verified customer

Asked: 2017-07-10

Answer

The products we sell, including anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-07-10

Question

Please see the WB image, lot number and protocol we used for thymus using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550. Please let me know if you require anything else.

R. Wu

Verified customer

Asked: 2016-07-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-07-11

Question

I have a question about product A02236-Dyl550, anti-Mouse CD8 alpha DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Parker

Verified customer

Asked: 2016-03-17

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-03-17

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A02236-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$330.00

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