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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-RNF43 Antibody Picoband™
Boster Bio Anti-RNF43 Antibody Picoband™ catalog # A01694-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF43 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01694-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01694-1 is reactive to RNF43 in Mouse, Rat
A01694-1 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RNF43 using anti-RNF43 antibody (A01694-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF43 antigen affinity purified polyclonal antibody (Catalog # A01694-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF43 at approximately 86KD. The expected band size for RNF43 is at 86KD.
Gene/Protein Information For RNF43 (Source: Uniprot.Org, NCBI)
E3 ubiquitin-protein ligase RNF43
E3 ubiquitin-protein ligase RNF43 RNF43 RNF124, SSPCS, URCC ring finger protein 43 E3 ubiquitin-protein ligase RNF43|RING-type E3 ubiquitin transferase RNF43*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RNF43, check out the RNF43 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RNF43: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A01694-1
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