Anti-RNF43 Antibody Picoband®

RNF43 antibody

Boster Bio Anti-RNF43 Antibody Picoband® catalog # A01694-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01694-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-RNF43 Antibody Picoband®

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SKU/Catalog Number

A01694-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RNF43 Antibody Picoband® catalog # A01694-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNF43 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01694-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01694-1 is reactive to RNF43 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

86 kDa

Calculated molecular weight

85.722kDa

Background of RNF43

RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01694-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml
ELISA,0.1-0.5μg/ml

Positive Control

WB: human Hela whole cell, human 293T whole cell, human COLO-320 whole cell, human SW620 whole cell, human MCF-7 whole cell

Validation Images & Assay Conditions

Gene/Protein Information For RNF43 (Source: Uniprot.org, NCBI)

Gene Name

RNF43

Full Name

E3 ubiquitin-protein ligase RNF43

Weight

85.722kDa

Superfamily

ZNRF3 family

Alternative Names

E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RING-type E3 ubiquitin transferase RNF43; RNF43 RNF43 RNF124, SSPCS, URCC ring finger protein 43 E3 ubiquitin-protein ligase RNF43|RING-type E3 ubiquitin transferase RNF43

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RNF43, check out the RNF43 Infographic

RNF43 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RNF43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-RNF43 Antibody Picoband®

Question

Is there a BSA free version of anti-RNF43 antibody A01694-1 available?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RNF43 antibody A01694-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-29

Question

Was western blot performed on cell lysates without exogenous overexpression of the genes for A01694-1?

Verified customer

Asked: 2019-05-03

Answer

The western blot on the Anti-RNF43 Antibody Picoband™ (A01694-1) product page was indeed performed on cell lysates without exogenous overexpression of the genes.

Boster Scientific Support

Answered: 2019-05-06

Question

We are currently using anti-RNF43 antibody A01694-1 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-23

Answer

The anti-RNF43 antibody (A01694-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-23

Question

Can you help my question with product A01694-1, anti-RNF43 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-04

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01694-1 anti-RNF43 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-04

Order DetailsPrice
A01694-1

100μg

$370
A01694-1-10ug

10μg sample (liquid)

$99
A01694-1-Biotin

100 μg Biotin conjugated

$570
A01694-1-Cy3

100 μg Cy3 conjugated

$570
A01694-1-Dylight488

100 μg Dylight488 conjugated

$570
A01694-1-Dylight550

100 μg Dylight550 conjugated

$570
A01694-1-Dylight594

100 μg Dylight594 conjugated

$570
A01694-1-FITC

100 μg FITC conjugated

$570
A01694-1-HRP

100 μg HRP conjugated

$570
A01694-1-APC

100 μg APC conjugated

$670
A01694-1-PE

100 μg PE conjugated

$670
A01694-1-iFluor647

100 μg iFluor647 conjugated

$670
A01694-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A01694-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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