|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-ROBO1 Picoband™ Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for ROBO1 detection. Tested with WB, Direct ELISA in Human;Mouse;Rat.|
|Cite This Product||Anti-ROBO1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01530-2)|
|Contents/Buffer||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human ROBO1 recombinant protein (Position: F264-P897).|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||roundabout guidance receptor 1|
|Tissue Specificity||Widely expressed, with exception of kidney.|
|Alternative Names||Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1|
|Subcellular Localization||Cell membrane. Single-pass type I membrane protein. Axon. Endoplasmic reticulum-Golgi intermediate compartment membrane. Single-pass membrane protein.|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.|
|Background||Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.|
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