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Product Info Summary
| SKU: | A00722 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ROCK1 Antibody
SKU/Catalog Number
A00722
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ROCK1 Antibody (Catalog # A00722). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ROCK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ROCK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00722)
Host
Rabbit
Contents
ROCK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1. The immunogen is located within the first 50 amino acids of ROCK1.
Cross-reactivity
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Reactive Species
A00722 is reactive to ROCK1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
158.2 kDa
Background of ROCK1
ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00722 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 μg/mL.
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Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 μg/mL.
Specific Publications For Anti-ROCK1 Antibody (A00722)
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