Anti-ROCK1 Antibody

ROCK1 antibody

Boster Bio Anti-ROCK1 Antibody (Catalog # A00722). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00722
Size: 0.1 mg
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

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Product Name

Anti-ROCK1 Antibody

View all ROCK1 Antibodies

SKU/Catalog Number

A00722

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ROCK1 Antibody (Catalog # A00722). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ROCK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ROCK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00722)

Host

Rabbit

Contents

ROCK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

SK7

Isotype

IgG

Immunogen

ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1. The immunogen is located within the first 50 amino acids of ROCK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.

Reactive Species

A00722 is reactive to ROCK1 in Human, Mouse, Rat

Applications

A00722 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

158.175kDa

Background of ROCK1

ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For ROCK1 (Source: Uniprot.org, NCBI)

Gene Name

ROCK1

Full Name

Rho-associated protein kinase 1

Weight

158.175kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.1; MGC131603; MGC43611; p160 ROCK-1; p160ROCK; p160-ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; ROCK1; ROK beta ROCK1 P160ROCK, ROCK-I Rho associated coiled-coil containing protein kinase 1 rho-associated protein kinase 1|p160 ROCK-1|renal carcinoma antigen NY-REN-35

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ROCK1, check out the ROCK1 Infographic

ROCK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ROCK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00722-0.1 mg

0.1 mg

$449

Free Secondary Antibody

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