Product Info Summary
SKU: | A11611 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-RPC4 POLR3D Antibody
SKU/Catalog Number
A11611
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RPC4 POLR3D Antibody (Catalog # A11611). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPC4 POLR3D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11611)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPC4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11611 is reactive to POLR3D in Human
Applications
A11611 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.396kDa
Background of POLR3D
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity.
Ittmann M., Mol. Cell. Biol. 7:3386-3393(1987).
Hu P., Mol. Cell. Biol. 22:8044-8055(2002).
Kalnine N., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from MCF-7 cells, using RPC4 antibody A11611.
Protein Target Info & Infographic
Gene/Protein Information For POLR3D (Source: Uniprot.org, NCBI)
Gene Name
POLR3D
Full Name
DNA-directed RNA polymerase III subunit RPC4
Weight
44.396kDa
Superfamily
eukaryotic RPC4/POLR3D RNA polymerase subunit family
Alternative Names
BN51 (BHK21) temperature sensitivity complementing; BN51; BN51TDNA-directed RNA polymerase III 47 kDa polypeptide; DNA-directed RNA polymerase III subunit D; DNA-directed RNA polymerase III subunit RPC4; polymerase (RNA) III (DNA directed) polypeptide D, 44kDa; Protein BN51; RNA polymerase III 47 kDa subunit; RNA polymerase III subunit C4; RPC4; RPC53 homolog; temperature sensitive complementation, cell cycle specific, tsBN51; TSBN51RPC53 POLR3D BN51T, C53, RPC4, RPC53, TSBN51 RNA polymerase III subunit D DNA-directed RNA polymerase III subunit RPC4|BN51 (BHK21) temperature sensitivity complementing|DNA-directed RNA polymerase III 47 kDa polypeptide|DNA-directed RNA polymerase III subunit D|RNA polymerase III 47 kDa subunit|RNA polymerase III subunit C4|RPC53 homolog|polymerase (RNA) III (DNA directed) polypeptide D, 44kDa|polymerase (RNA) III subunit D|temperature sensitive complementation, cell cycle specific, tsBN51
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR3D, check out the POLR3D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPC4 POLR3D Antibody (A11611)
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No publications found for A11611
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-RPC4 POLR3D Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-RPC4 antibody A11611. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-30
Question
Does anti-RPC4 antibody A11611 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
According to the expression profile of cervix carcinoma erythroleukemia, POLR3D is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-RPC4 antibody A11611 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-05-07
Question
Will A11611 anti-RPC4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
It shows on the product datasheet, A11611 anti-RPC4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-23
Question
Is there a BSA free version of anti-RPC4 antibody A11611 available?
M. Li
Verified customer
Asked: 2015-12-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RPC4 antibody A11611 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-12-09