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Product Info Summary
| SKU: | A06803-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-RPL23A Antibody (C-term)
SKU/Catalog Number
A06803-1
Size
80 µl
Description
Boster Bio Anti-RPL23A Antibody (C-term) (Catalog # A06803-1). Tested in IHC-P, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RPL23A Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06803-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RPL23A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human RPL23A.
Reactive Species
A06803-1 is reactive to RPL23A in Human, Mouse, Rat
Calculated molecular weight
17.7 kDa
Background of RPL23A
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL23A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. This gene is co-transcribed with the U42A, U42B, U101A, and U101B small nucleolar RNA genes, which are located in its third, first, second, and fourth introns, respectively.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06803-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:100
Validation Images & Assay Conditions
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All lanes : Anti-RPL23A Antibody (C-term) at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: 293T/17 whole cell lysate
Lane 5: Mouse heart tissue lysate
Lane 6: Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 18 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of RPL23A Antibody (C-term) on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of A06803-1 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-RPL23A Antibody (C-term) (A06803-1)
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