Overview
| Product Name |
Anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal Antibody
See more RPS3 products |
| Catalog# |
M01542 |
| Pack Size |
100ug/vial |
| Storage & Handling |
At -20°C for one year. Avoid repeated freezing and thawing. |
| Description |
Monoclonal antibody for RIBOSOMAL PROTEIN S3/RPS3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat RIBOSOMAL PROTEIN S3/RPS3 information: Molecular Weight: 26688 MW; Subcellular Localization: Cytoplasm . Nucleus . Nucleus, nucleolus . Mitochondrion inner membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, spindle . In normal cells, located mainly in the cytoplasm with small amounts in the nucleus but translocates to the nucleus in cells undergoing apoptosis (By similarity). Nuclear translocation is induced by DNA damaging agents such as hydrogen peroxide (PubMed:17560175). Accumulates in the mitochondrion in response to increased ROS levels (PubMed:23911537). Localizes to the spindle during mitosis (PubMed:23131551). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:17289661). |
| Cite This Product |
Anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01542)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
IF, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A synthesized peptide derived from human RPS3 |
Gene/Protein Basic Information For RPS3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P23396 |
|---|
| NCBI Gene Id | 6188 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | RPS3 |
|---|
| Protein Name | 40S ribosomal protein S3 |
|---|
| Superfamily | universal ribosomal protein uS3 family |
|---|
| Alternative Names | RPS3|40S ribosomal protein S3; FLJ26283; FLJ27450; IMR-90 ribosomal protein S3; MGC87870; ribosomal protein S3 |
|---|
| Molecular Weight | 26688 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RPS3, check out the RPS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for RPS3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the RPS3 infographic
Our Boster Quality Guarantee for Anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal Antibody covers its use in the following applications.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Immunohistochemical analysis of paraffin-embedded human colon cancer, using RPS3 Antibody(M01542)
RPS3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RPS3 Antibody (M01542)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of RPS3 expression in HepG2 cell lysate (M01542).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS3 monoclonal antibody (Catalog # M01542) overnight at 4?? then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS3
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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Total number of citations: 0
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Questions and answers from customers related to M01542 Anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal Antibody
4 Related Questions
Question
I see that the anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal antibody M01542 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2019-07-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal antibody M01542. Let me know if you need anything else.
Verified Customer
Asked: 2017-12-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-22
Question
Is this M01542 anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal antibody reactive to the isotypes of RPS3?
Verified Customer
Asked: 2017-10-17
Answer
The immunogen of M01542 anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal antibody is A synthesized peptide derived from human RPS3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-17
Question
I am interested in to test anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal antibody M01542 on human lymph for research purposes, then I may be interested in using anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal antibody M01542 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Answer
The products we sell, including anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal antibody M01542, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-14
