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Product Info Summary
| SKU: | M01764 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RRM1 Monoclonal Antibody
SKU/Catalog Number
M01764
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RRM1 Monoclonal Antibody catalog # M01764. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RRM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01764)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFDH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RRM1 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01764 is reactive to RRM1 in Human, Mouse, Rat
Applications
M01764 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
90.07kDa
Background of RRM1
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of RRM1 expression in A549 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RRM1 (Source: Uniprot.org, NCBI)
Gene Name
RRM1
Full Name
Ribonucleoside-diphosphate reductase large subunit
Weight
90.07kDa
Superfamily
ribonucleoside diphosphate reductase large chain family
Alternative Names
EC 1.17.4.1; R1; ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; ribonucleotide reductase M1 polypeptide; ribonucleotide reductase M1; ribonucleotide reductase, large subunit; ribonucleotide reductase, R1 subunit; RIR1; RR1 RRM1 R1, RIR1, RR1 ribonucleotide reductase catalytic subunit M1 ribonucleoside-diphosphate reductase large subunit|ribonucleoside-diphosphate reductase subunit M1|ribonucleotide reductase M1 polypeptide|ribonucleotide reductase, R1 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRM1, check out the RRM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RRM1 Monoclonal Antibody (M01764)
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No publications found for M01764
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-RRM1 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01764?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of RRM1 expression in A549 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-RRM1 Monoclonal antibody M01764 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The anti-RRM1 Monoclonal antibody (M01764) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-26
Question
Can you help my question with product M01764, anti-RRM1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01764 anti-RRM1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-10
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-RRM1 Monoclonal antibody M01764. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-16
Question
Will M01764 anti-RRM1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Moore
Verified customer
Asked: 2016-02-19
Answer
As indicated on the product datasheet, M01764 anti-RRM1 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-02-19
