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Product Info Summary
| SKU: | A12453 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Rubicon RUBCN Antibody
SKU/Catalog Number
A12453
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Rubicon RUBCN Antibody (Catalog # A12453). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Rubicon RUBCN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12453)
Host
Rabbit
Contents
Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
Reactive Species
A12453 is reactive to RUBCN in Human, Mouse, Rat
Applications
A12453 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
108.622kDa
Background of RUBCN
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.
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Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 μg/ml.
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Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For RUBCN (Source: Uniprot.org, NCBI)
Gene Name
RUBCN
Full Name
Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
Weight
108.622kDa
Alternative Names
Run domain Beclin-1-interacting and cysteine-rich domain-containing protein RUBCN KIAA0226, RUBICON, SCAR15 rubicon autophagy regulator run domain Beclin-1-interacting and cysteine-rich domain-containing protein|RUN and cysteine rich domain containing beclin 1 interacting protein|RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein|baron|beclin-1 associated RUN domain containing protein|rundataxin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RUBCN, check out the RUBCN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RUBCN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rubicon RUBCN Antibody (A12453)
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No publications found for A12453
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Customer Reviews
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