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Product Info Summary
| SKU: | M00086 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody
View all RUNX1/CBFA2 Antibodies
SKU/Catalog Number
M00086
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody catalog # M00086. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00086)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOG-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RUNX1/RUNX2/RUNX3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00086 is reactive to RUNX1 in Human, Mouse, Rat
Applications
M00086 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
48.737kDa
Background of RUNX1/CBFA2
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of RUNX1/RUNX2/RUNX3 expression in MOLT4 cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse lung, using RUNX1/RUNX2/RUNX3 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For RUNX1 (Source: Uniprot.org, NCBI)
Gene Name
RUNX1
Full Name
Runt-related transcription factor 1
Weight
48.737kDa
Alternative Names
Acute myeloid leukemia 1 protein; AML1; AML1acute myeloid leukemia 1; AML1-EVI-1; CBFA2; CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1; Core-binding factor subunit alpha-2; EVI-1; PEBP2A2; runt domain, alpha subunit 2; runt-related transcription factor 1; RUNX1; SL3/AKV core-binding factor alpha B subunit RUNX1 AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha RUNX family transcription factor 1 runt-related transcription factor 1|AML1-EVI-1 fusion protein|PEA2-alpha B|PEBP2-alpha B|SL3-3 enhancer factor 1 alpha B subunit|SL3/AKV core-binding factor alpha B subunit|acute myeloid leukemia 1 protein|core-binding factor, runt domain, alpha subunit 2|mutant RUNX1|oncogene AML-1|polyomavirus enhancer-binding protein 2 alpha B subunit|runt related transcription factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RUNX1, check out the RUNX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RUNX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody (M00086)
Hello CJ!
No publications found for M00086
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody
Question
We are currently using anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody (M00086) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-02
Question
We want to test anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The products we sell, including anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-30
Question
Our team were happy with the WB result of your anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody. However we have been able to see positive staining in monocyte nucleus. using this antibody. Is that expected? Could you tell me where is RUNX1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
From literature, monocyte does express RUNX1. Generally RUNX1 expresses in nucleus. Regarding which tissues have RUNX1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7891692
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Leukocyte, Pubmed ID: 1720541
Liver, Pubmed ID: 24275569
Monocyte, Pubmed ID: 7835892, 8634147
Peripheral blood, Pubmed ID: 8395054
Boster Scientific Support
Answered: 2020-01-29
Question
I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-30
Question
Does anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 work on zebrafish IF with liver?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Our lab technicians have not tested anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Would M00086 anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
As indicated on the product datasheet, M00086 anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-03
Question
Can you help my question with product M00086, anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00086 anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-02
Question
Is this M00086 anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody reactive to the isotypes of RUNX1?
B. Walker
Verified customer
Asked: 2019-05-21
Answer
The immunogen of M00086 anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody is A synthesized peptide derived from human RUNX1/RUNX2/RUNX3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-21
Question
I was wanting to use your anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody for IF for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
You can see on the product datasheet, M00086 anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-23
Question
Do you have a BSA free version of anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 available?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-23
Question
Would anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
According to the expression profile of leukemic t-cell, RUNX1 is highly expressed in leukemic t-cell. So, it is likely that anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2017-09-28
Question
Our lab used your anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody for IF on monocyte in the past. I am using human, and We are going to use the antibody for IP next. Our lab want to know about examining monocyte as well as erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IP?
R. Parker
Verified customer
Asked: 2017-01-31
Answer
I took a look at the website and datasheets of our anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody and I see that M00086 has been validated on human in both IF and IP. Thus M00086 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-01-31
Question
Is a blocking peptide available for product anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody (M00086)?
C. Evans
Verified customer
Asked: 2014-12-12
Answer
We do provide the blocking peptide for product anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody (M00086). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-12
Question
I see that the anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody M00086 works with IF, what is the protocol used to produce the result images on the product page?
L. Krishna
Verified customer
Asked: 2014-06-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-06-26
Question
We have been able to see staining in rat cervix carcinoma. What should we do? Is anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
R. Krishna
Verified customer
Asked: 2013-05-03
Answer
Based on literature cervix carcinoma does express RUNX1. Based on Uniprot.org, RUNX1 is expressed in epithelium of mammary gland, leukocyte, monocyte, b-cell, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, peripheral blood, among other tissues. Regarding which tissues have RUNX1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7891692
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Leukocyte, Pubmed ID: 1720541
Liver, Pubmed ID: 24275569
Monocyte, Pubmed ID: 7835892, 8634147
Peripheral blood, Pubmed ID: 8395054
Boster Scientific Support
Answered: 2013-05-03
