Anti-RUNX2 Monoclonal Antibody

Product Name

Anti-RUNX2 Monoclonal Antibody

View all RUNX2/CBFA1 Antibodies

SKU/Catalog Number

M00442

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-RUNX2 Monoclonal Antibody catalog # M00442. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RUNX2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00442)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HCO-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human RUNX2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00442 is reactive to RUNX2 in Human, Mouse, Rat

Applications

M00442 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

57 kDa

Calculated molecular weight

56.648kDa

Background of RUNX2/CBFA1

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RUNX2 using anti-RUNX2 antibody (M00442).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified monoclonal antibody (Catalog # M00442) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 57 kDa. The expected band size for RUNX2 is at 57 kDa.

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Immunohistochemical analysis of paraffin-embedded human colon, using RUNX2 Antibody.

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Immunofluorescent analysis of Saos-2 cells, using RUNX2 Antibody.

Gene/Protein Information For RUNX2 (Source: Uniprot.org, NCBI)

Alternative Names

Acute myeloid leukemia 3 protein; AML3CCD; CBFA1; CBFA1MGC120022; Core-binding factor subunit alpha-1; MGC120023; PEA2-alpha A; PEBP2A; PEBP2A1CCD1; PEBP2aA1; PEBP2-alpha A; runt domain, alpha subunit 1; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit RUNX2 AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA RUNX family transcription factor 2 runt-related transcription factor 2|PEA2-alpha A|PEBP2-alpha A|SL3-3 enhancer factor 1 alpha A subunit|SL3/AKV core-binding factor alpha A subunit|acute myeloid leukemia 3 protein|core-binding factor, runt domain, alpha subunit 1|oncogene AML-3|osteoblast-specific transcription factor 2|polyomavirus enhancer-binding protein 2 alpha A subunit|runt related transcription factor 2

For more info on RUNX2, check out the RUNX2 Infographic

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In this infographic, you will see the following information for RUNX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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