Anti-RUSC1 Antibody

Product Name

Anti-RUSC1 Antibody

View all RUSC1 Antibodies

SKU/Catalog Number

A11204

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RUSC1 Antibody (Catalog # A11204). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RUSC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RUSC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11204)

Host

Rabbit

Contents

RUSC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RUSC1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RUSC1. The immunogen is located within amino acids 780 - 830 of RUSC1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.

Reactive Species

A11204 is reactive to RUSC1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

96444 MW

Background of RUSC1

RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11204 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For RUSC1 (Source: Uniprot.org, NCBI)

Alternative Names

NESCA, NESCA, RUN and SH3 domain-containing protein 1, New molecule containing SH3 at the carboxy-terminus, Nesca RUSC1 NESCA RUN and SH3 domain containing 1 RUN and SH3 domain-containing protein 1|new molecule containing SH3 at the carboxy-terminus

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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