Anti-RuvB-like 1 RUVBL1 Antibody

RUVBL1 antibody

Boster Bio Anti-RuvB-like 1 RUVBL1 Antibody (Catalog# A02049). Tested in IHC, WB application(s). This antibody reacts with Human.

Product Info Summary

SKU: A02049
Size: 100 µg
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-RuvB-like 1 RUVBL1 Antibody

View all RUVBL1 Antibodies

SKU/Catalog Number

A02049

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-RuvB-like 1 RUVBL1 Antibody (Catalog# A02049). Tested in IHC, WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-RuvB-like 1 RUVBL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02049)

Host

Rabbit

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Polyclonal

Clone Number

CFA-18

Isotype

Rabbit IgG

Immunogen

A partial length recombinant RuvB-like 1 protein (amino acids 1-268)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, horse, rhesus macaque, dog, chimpanzee.

Reactive Species

A02049 is reactive to RUVBL1 in Human

Applications

A02049 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.228kDa

Background of RUVBL1

NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 0.5-1 µg/ml
IHC: 10 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For RUVBL1 (Source: Uniprot.org, NCBI)

Gene Name

RUVBL1

Full Name

RuvB-like 1

Weight

50.228kDa

Superfamily

RuvB family

Alternative Names

49 kDa TATA box-binding protein-interacting protein; 54 kDa erythrocyte cytosolic protein; EC 3.6.1; EC 3.6.4.12,49 kDa TBP-interacting protein; ECP54; INO80 complex subunit H; INO80HTIP49A; NMP 238; NMP238ECP-54; Nuclear matrix protein 238; Pontin 52; PONTIN; Pontin52; RuvB (E coli homolog)-like 1; RuvB-like 1 (E. coli); ruvB-like 1; RVB1; TATA binding protein interacting protein 49 kDa; TIH1; TIP49a; TIP49TAP54-alpha; TIP60-associated protein 54-alpha RUVBL1 ECP-54, ECP54, INO80H, NMP 238, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A RuvB like AAA ATPase 1 ruvB-like 1|49 kDa TATA box-binding protein-interacting protein|49 kDa TBP-interacting protein|54 kDa erythrocyte cytosolic protein|INO80 complex subunit H|RuvB (E coli homolog)-like 1|RuvB-like AAA ATPase|TAP54-alpha|TATA binding protein interacting protein 49 kDa|TIP60-associated protein 54-alpha|epididymis secretory sperm binding protein|nuclear matrix protein 238|pontin 52

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RUVBL1, check out the RUVBL1 Infographic

RUVBL1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RUVBL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-RuvB-like 1 RUVBL1 Antibody

Question

I see that the anti-RuvB-like 1 antibody A02049 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-15

Question

Is there a BSA free version of anti-RuvB-like 1 antibody A02049 available?

Verified Customer

Verified customer

Asked: 2019-02-15

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RuvB-like 1 antibody A02049 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-02-15

Question

Is this A02049 anti-RuvB-like 1 antibody reactive to the isotypes of RUVBL1?

K. Patel

Verified customer

Asked: 2018-01-04

Answer

The immunogen of A02049 anti-RuvB-like 1 antibody is A partial length recombinant RuvB-like 1 protein (amino acids 1-268). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-04

Question

We are currently using anti-RuvB-like 1 antibody A02049 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

The anti-RuvB-like 1 antibody (A02049) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-15

Question

I was wanting to use your anti-RuvB-like 1 antibody for IHC for human bone marrow on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bone marrow identification?

T. Jackson

Verified customer

Asked: 2015-09-08

Answer

As indicated on the product datasheet, A02049 anti-RuvB-like 1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-09-08

Question

See below the WB image, lot number and protocol we used for bone marrow using anti-RuvB-like 1 antibody A02049. Please let me know if you require anything else.

M. Edwards

Verified customer

Asked: 2014-03-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-03-18

Order DetailsPrice
A02049-100ug

100 μg

$341

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