This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
S100A12 antibody (A01478-1) performs well in IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of S100A12 using anti-S100A12 antibody (A01478-1).
S100A12 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A12 Antibody (A01478-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
S100A12 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A12 Antibody (A01478-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-S100A12 Antibody
S100A12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC |
Tested Applications
S100A12 antibody was positively detected by IHC in:
human spleen tissue, human spleen tissue, human spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
1-2 µg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
IHC
IF
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-S100A12 Antibody
View all S100A12 Antibodies
View all S100A12 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human S100A12.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Calculated Molecular Weight
10.6 kDa
Background of S100A12
S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity.
Cite This Product
Anti-S100A12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01478-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
91%
High Homology
Rabbit
55%
Low homology
Dog
50%
Low homology
Cow
45%
Low homology
Sheep
45%
Low homology
Mouse
32%
Low homology
Rat
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for S100A12 antibody
Review
Have you used Anti-S100A12 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-S100A12 Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01478-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026