Anti-S100A4 (Marker of Tumor Metastasis) Monoclonal Antibody

Product Name

Anti-S100A4 (Marker of Tumor Metastasis) Monoclonal Antibody

View all S100A4 Antibodies

SKU/Catalog Number

M01217

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-S100A4 (Marker of Tumor Metastasis) Monoclonal Antibody (Catalog # M01217). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-S100A4 (Marker of Tumor Metastasis) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01217)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: S100A4/1482

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (around aa 1-101) of human S100A4 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01217 is reactive to S100A4 in Human, Mouse

Applications

M01217 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

11.729kDa

Background of S100A4

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, α6β4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-S100A4 Mouse Monoclonal Antibody (S100A4/1482).

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Western Blot of HeLa, A549 and A375 cell lysate using Anti-S100A4 Mouse Monoclonal Antibody (S100A4/1482).

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Western Blot analysis of HeLa cell lysate using Anti-S100A4 Mouse Monoclonal Antibody (S100A4/1482).

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Flow Cytometric analysis of T98G cells using Anti-S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Flow Cytometric analysis of A549 cells using Anti-S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Confocal immunofluorescence image of HeLa Cells using Anti-S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.

Gene/Protein Information For S100A4 (Source: Uniprot.org, NCBI)

Alternative Names

18A2; 42A; Calvasculin; CAPL; CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin; fibroblast-specific protein-1,42A; FSP1; leukemia multidrug resistance associated protein; malignant transformation suppression 1; Metastasin; MTS1; murine placental homolog); P9KA; PEL98; Placental calcium-binding protein; Protein Mts1; protein S100-A4; S100 calcium binding protein A4; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium-binding protein A4; S100A4 S100A4 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98 S100 calcium binding protein A4 protein S100-A4|S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)|calcium placental protein|fibroblast-specific protein-1|leukemia multidrug resistance associated protein|malignant transformation suppression 1|metastasin 1|murine placental homolog|placental calcium-binding protein|protein Mts1

For more info on S100A4, check out the S100A4 Infographic

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In this infographic, you will see the following information for S100A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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