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Product Info Summary
| SKU: | A02904-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, ICC, WB |
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Product info
Product Name
Anti-S1PR2 Antibody
SKU/Catalog Number
A02904-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-S1PR2 Antibody catalog # A02904-2. Tested in WB, ICC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-S1PR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02904-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human S1PR2 recombinant protein (Position: N280-T351).
Reactive Species
A02904-2 is reactive to S1PR2 in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
38.9 kDa
Background of S1PR2
The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02904-2 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-400
Immunoprecipitation, 1:50
ELISA, 1:100-1000
Positive Control
WB: human Hela whole cell, human HUH-7 whole cell, human MCF-7 whole cell, human MDA-MB-231 whole cell
Validation Images & Assay Conditions
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Western blot analysis of S1PR2 using anti-S1PR2 antibody (A02904-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HUH-7 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human MDA-MB-231 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S1PR2 antigen affinity purified polyclonal antibody (A02904-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for S1PR2 at approximately 45 kDa. The expected band size for S1PR2 is at 39 kDa.
Specific Publications For Anti-S1PR2 Antibody (A02904-2)
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