Click image to see more details
Product Info Summary
| SKU: | M01475-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01475-1
BM4200 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody catalog # M01475-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01475-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CDB-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human S6K1
Reactive Species
M01475-1 is reactive to RPS6KB1 in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01475-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human 293T whole cell, human MCF-7 whole cell, human HepG2 whole cell, human Hela whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of p70 S6K/RPS6KB1 using anti-p70 S6K/RPS6KB1 antibody (M01475-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p70 S6K/RPS6KB1 antigen affinity purified monoclonal antibody (M01475-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p70 S6K/RPS6KB1 at approximately 59 kDa. The expected band size for p70 S6K/RPS6KB1 is at 59 kDa.
Specific Publications For Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody (M01475-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-S6K1 Rabbit Monoclonal antibody M01475-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-07
Question
Does anti-S6K1 Rabbit Monoclonal antibody M01475-1 work on monkey IP with cerebellar vermis?
K. Zhao
Verified customer
Asked: 2018-09-24
Answer
Our lab technicians have not tested anti-S6K1 Rabbit Monoclonal antibody M01475-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-S6K1 Rabbit Monoclonal antibody M01475-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cerebellar vermis in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-24
Question
Would anti-S6K1 Rabbit Monoclonal antibody M01475-1 work for WB with cervix carcinoma?
Z. Lewis
Verified customer
Asked: 2018-04-13
Answer
According to the expression profile of cervix carcinoma, RPS6KB1 is highly expressed in cervix carcinoma. So, it is likely that anti-S6K1 Rabbit Monoclonal antibody M01475-1 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-04-13
Question
I was wanting to use your anti-S6K1 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
It shows on the product datasheet, M01475-1 anti-S6K1 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-26
Question
Is a blocking peptide available for product anti-S6K1 Rabbit Monoclonal antibody (M01475-1)?
L. Anderson
Verified customer
Asked: 2017-07-17
Answer
We do provide the blocking peptide for product anti-S6K1 Rabbit Monoclonal antibody (M01475-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-17
Question
I am looking for to test anti-S6K1 Rabbit Monoclonal antibody M01475-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-S6K1 Rabbit Monoclonal antibody M01475-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Li
Verified customer
Asked: 2016-05-24
Answer
The products we sell, including anti-S6K1 Rabbit Monoclonal antibody M01475-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-24
