Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody

p70 S6 Kinase/S6K antibody

Boster Bio Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody catalog # M01475-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01475-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IHC, WB

Product Name

Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody

View all p70 S6 Kinase/S6K Antibodies

SKU/Catalog Number

M01475-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody catalog # M01475-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01475-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CDB-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human S6K1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01475-1 is reactive to RPS6KB1 in Human, Mouse, Rat

Applications

M01475-1 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

59.14kDa

Background of p70 S6 Kinase/S6K

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For RPS6KB1 (Source: Uniprot.org, NCBI)

Gene Name

RPS6KB1

Full Name

Ribosomal protein S6 kinase beta-1

Weight

59.14kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A RPS6KB1 PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha ribosomal protein S6 kinase B1 ribosomal protein S6 kinase beta-1|ribosomal protein S6 kinase I|ribosomal protein S6 kinase, 70kDa, polypeptide 1|serine/threonine kinase 14 alpha|serine/threonine-protein kinase 14A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPS6KB1, check out the RPS6KB1 Infographic

RPS6KB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPS6KB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-S6K1 Rabbit Monoclonal antibody M01475-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-07

Question

Does anti-S6K1 Rabbit Monoclonal antibody M01475-1 work on monkey IP with cerebellar vermis?

K. Zhao

Verified customer

Asked: 2018-09-24

Answer

Our lab technicians have not tested anti-S6K1 Rabbit Monoclonal antibody M01475-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-S6K1 Rabbit Monoclonal antibody M01475-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cerebellar vermis in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-24

Question

Would anti-S6K1 Rabbit Monoclonal antibody M01475-1 work for WB with cervix carcinoma?

Z. Lewis

Verified customer

Asked: 2018-04-13

Answer

According to the expression profile of cervix carcinoma, RPS6KB1 is highly expressed in cervix carcinoma. So, it is likely that anti-S6K1 Rabbit Monoclonal antibody M01475-1 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2018-04-13

Question

I was wanting to use your anti-S6K1 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

It shows on the product datasheet, M01475-1 anti-S6K1 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-26

Question

Is a blocking peptide available for product anti-S6K1 Rabbit Monoclonal antibody (M01475-1)?

L. Anderson

Verified customer

Asked: 2017-07-17

Answer

We do provide the blocking peptide for product anti-S6K1 Rabbit Monoclonal antibody (M01475-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-07-17

Question

I am looking for to test anti-S6K1 Rabbit Monoclonal antibody M01475-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-S6K1 Rabbit Monoclonal antibody M01475-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Li

Verified customer

Asked: 2016-05-24

Answer

The products we sell, including anti-S6K1 Rabbit Monoclonal antibody M01475-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-05-24

Order DetailsPrice
M01475-1

100μL

$370
M01475-1-30ul

30μL sample

$99
M01475-1-carrier-free

Carrier Free

$370

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M01475-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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