Product Info Summary
SKU: | A04756-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-sarcoglycan Alpha SGCA Antibody
View all alpha-Sarcoglycan Antibodies
SKU/Catalog Number
A04756-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-sarcoglycan Alpha SGCA Antibody catalog # A04756-1. Tested in WB,IP,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-sarcoglycan Alpha SGCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04756-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PAIRB protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04756-1 is reactive to SGCA in Human, Mouse, Rat
Applications
A04756-1 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42.875kDa
Background of alpha-Sarcoglycan
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.
Walter Makous, Invest. Ophthalmol. Vis. Sci., Sep 2006; 47: 4160 - 4167.
Hsin-Pei Shih, J. Cell Sci., Mar 2002; 115: 1259.
Thomas Buschmann, J. Biol. Chem., Oct 2001; 276: 40389 - 40395.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
IP: 1:50-1:100
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of SGCA using Anti-sarcoglycan Alpha SGCA Antibody (A04756-1).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-sarcoglycan Alpha SGCA Antibody A04756-1
Western blot analysis of alpha sarcoglycan on rat heart cells lysates using anti-alpha sarcoglycan antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SGCA (Source: Uniprot.org, NCBI)
Gene Name
SGCA
Full Name
Alpha-sarcoglycan
Weight
42.875kDa
Superfamily
sarcoglycan alpha/epsilon family
Alternative Names
50-DAG; Adhalin; ADL; alphaSarcoglycan; alpha-Sarcoglycan; DAG2; DMDA2; DMDA250kD DAG; Dystroglycan-2; LGMD2D; LGMD2Dalpha (50kD dystrophin-associated glycoprotein); sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein); SCARMD1; SGCA SGCA 50DAG, ADL, DAG2, DMDA2, LGMD2D, LGMDR3, SCARMD1, adhalin sarcoglycan alpha alpha-sarcoglycan|50 kDa dystrophin-associated glycoprotein|50kD DAG|alpha-SG|dystroglycan-2|limb girdle muscular dystrophy 2D|sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SGCA, check out the SGCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SGCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-sarcoglycan Alpha SGCA Antibody (A04756-1)
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No publications found for A04756-1
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Customer Reviews
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