Product Info Summary
| SKU: | A03633 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SARM SARM1 Antibody
SKU/Catalog Number
A03633
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SARM SARM1 Antibody (Catalog # A03633). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SARM antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SARM SARM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03633)
Host
Rabbit
Contents
SARM Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SARM antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human SARM. The immunogen is located within amino acids 640 - 690 of SARM.
Reactive Species
A03633 is reactive to SARM1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
79.4 kDa
Background of SARM1
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SARM (SAM and ARM-containing protein), along with other molecules such as TIRP, TRIF, TIRAP, and MyD88, is thought to serve as an adaptor protein for the TLRs that allows for the activation of downstream kinases and NF-κB, and ultimately the expression of proteins involved in host defense. While SARM has not been conclusively shown to associate directly with TLRs, the presence of a Toll-interluekin-1 (TIR) domain in SARM is consistent with a role as a signaling molecule.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03633 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SARM antibody can be used for detection of SARM by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SARM in Daudi cell lysate with SARM antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry staining of Daudi cells using SARM antibody at 2 μg/mL.
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Immunofluorescence of SARM in Daudi cells with SARM antibody at 2 μg/mL.
Specific Publications For Anti-SARM SARM1 Antibody (A03633)
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