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Scd1 antibody (M32769) performs well in Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of SCD1 using anti-SCD1 antibody (M32769).
SCD1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SCD1 Antibody (M32769) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SCD1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SCD1 Antibody (M32769) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-SCD1 Antibody (Monoclonal, 31S66)
Scd1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
Scd1 antibody was positively detected by IHC in:
mouse liver tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunohistochemistry
1:50-200
Flow Cytometry (Fixed)
1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Mouse
Recommend
Tested
Recommend
Recommend
Rat
Recommend
Tested
Recommend
Recommend
Product Information
Product Name
Anti-SCD1 Antibody (Monoclonal, 31S66)
View all SCD1 Antibodies
View all SCD1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31S66
Immunogen
Synthetic peptide within mouse SCD1 aa 3-48.
Calculated Molecular Weight
41.0 kDa
Background of Scd1
Enables metal ion binding activity; palmitoyl-CoA 9-desaturase activity; and stearoyl-CoA 9-desaturase activity. Involved in several processes, including positive regulation of cold-induced thermogenesis; sterol homeostasis; and tarsal gland development. Acts upstream of or within several processes, including brown fat cell differentiation; monounsaturated fatty acid biosynthetic process; and white fat cell differentiation. Located in endoplasmic reticulum membrane. Is expressed in several structures, including adipose tissue; central nervous system; cranium; integumental system; and liver. Orthologous to human SCD (stearoyl-CoA desaturase).
Cite This Product
Anti-SCD1 Antibody (Monoclonal, 31S66) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32769)
Protocols
Immunohistochemistry (IHC) protocol for Scd1 antibody
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Product Promise
We promise Anti-SCD1 Antibody (Monoclonal, 31S66) performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M32769
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026