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Product Info Summary
| SKU: | A01254 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SCF KITLG Antibody
SKU/Catalog Number
A01254
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SCF KITLG Antibody (Catalog # A01254). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SCF KITLG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01254)
Host
Rabbit
Contents
SCF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-SCF antibody was raised against a peptide corresponding to 18 amino acids near the center of human SCF. The immunogen is located within amino acids 100-150 of SCF.
Reactive Species
A01254 is reactive to KITLG in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
30.9 kDa
Background of KITLG
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01254 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Cell Lines and Tissues of Human, Mouse and Rat
Loading: 15 μg of lysates per lane.
Antibodies: SCF A01254 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of SCF in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCF antibody (A01254) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western Blot Validation with Recombinant Protein
Loading: 30 ng of human SCF recombinant protein per lane.
Antibodies: SCF A01254 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Observed at around 20kD.
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Immunofluorescence Validation of SCF in Human Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling SCF with A01254 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Regulation Validation of SCF in Streptozotocin (STZ)-induced Diabetic Mice (Zhang et al., 2016)
WB analysis showed protein expression level of SCF detected by anti-SCF antibody (A01254) in gastric smooth muscle was significantly decreased in STZ-induced diabetic mice as compared to the control.
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Regulated Expression Validation of SCF in Gastric Smooth Muscle Cells (GSMCs) of Mice (Zhang et al., 2016)
WB analysis of protein expression level of SCF detected by anti-SCF antibody (A01254). GSMCs were treated with PI3K inhibitor (LY294002), AT1R inhibitor (ZD7155) and AT2R inhibitor (PD123319) before Ang II treatment. Ang II (10-8mol/L) significantly increased SCF protein expression, which was reduced by treatment of LY294002 and ZD7155.
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Regulated Expression Validation of SCF in GSMCs of Normal Mice (Wu et al., 2013)
WB analysis of protein expression level of SCF detected by anti-SCF antibody (A01254). GSMCs were treated with C-type natriuretic peptide (CNP) at different doses for 48hr. CNP(10-7mol/L and 10-6mol/L) significantly decreased SCF protein expression level as compared to the control group.
Specific Publications For Anti-SCF KITLG Antibody (A01254)
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