Anti-SCRAPPER FBXL20 Antibody

Product Name

Anti-SCRAPPER FBXL20 Antibody

View all FBXL20 Antibodies

SKU/Catalog Number

A12714

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SCRAPPER FBXL20 Antibody (Catalog # A12714). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SCRAPPER antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SCRAPPER FBXL20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12714)

Host

Rabbit

Contents

SCRAPPER Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SCRAPPER antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human SCRAPPER. The immunogen is located within the last 50 amino acids of SCRAPPER.

Reactive Species

A12714 is reactive to FBXL20 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

48.4 kDa

Background of FBXL20

Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12714 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FBXL20

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