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Product Info Summary
| SKU: | A09179 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SCUBE2 Antibody
SKU/Catalog Number
A09179
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SCUBE2 Antibody (Catalog # A09179). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
SCUBE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SCUBE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09179)
Host
Rabbit
Contents
SCUBE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SCUBE2 antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2. The immunogen is located within amino acids 480 - 530 of SCUBE2.
Reactive Species
A09179 is reactive to SCUBE2 in Human, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
110.0 kDa
Background of SCUBE2
SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09179 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SCUBE2 antibody can be used for detection of SCUBE2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
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Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
Specific Publications For Anti-SCUBE2 Antibody (A09179)
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