Rabbit IgG polyclonal antibody for Succinate dehydrogenase[ubiquinone] flavoprotein subunit, mitochondrial(SDHA) detection. Tested with WB in Human;Mouse;Rat.
Anti-SDHA Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-SDHA Antibody
See all SDHA primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for SDHA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. SDHA information: Molecular Weight: 72692 MW; Subcellular Localization: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.|
|Cite This Product||Anti-SDHA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1717)|
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|Anti-SDHA Antibody may replace the following items: sc 27990|sc 98253|sc 166947|sc 377302|sc 27992|sc 166909|sc 390381.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human SDHA(113-128aa NMEEDNWRWHFYDTVK), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For SDHA (Source: Uniprot.org, NCBI)
|NCBI Gene Id||6389|
|Species Of This Entry||Human|
|Protein Name||Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial|
|Superfamily||FAD-dependent oxidoreductase 2 family|
|Alternative Names||SDHA|CMD1GG; EC 18.104.22.168; Fp; SDH1; SDH2; SDHFmitochondrial; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex, subunit A, flavoprotein (Fp)|
|Post Tranlational Modifications||Phosphorylation; Oxidation; Cleavage; Methylation; Deacetylation; Acetylation; Carboxylation; Reduction|
|Subcellular Localization||Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.|
|Gene Location||5p15.33, on Chromasome 5, gene sequence: NC_000005.10 (218223..264816)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SDHA (Source: Uniprot.org, NCBI)
|Protein Function||Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:24781757). Can act as a tumor suppressor (PubMed:20484225).|
|Interacting Proteins||PTPN3; SDHAF2; SDHB|
|Related Diseases||Neoplasms; Paraganglioma; Pheochromocytoma; Malignant Neoplasms; Von Hippel-lindau Syndrome; Neurofibromatosis 1; Adrenal Gland Neoplasms; Cell Transformation, Neoplastic; Carcinoma; Gastrointestinal Stromal Tumors; Malignant Paraganglionic Neoplasm; Hereditary Paraganglioma; Head And Neck Neoplasms; Neurofibromatoses; Hypoxia; Legionnaires' Disease; Endocrine Gland Neoplasms; Multiple Endocrine Neoplasia; Neoplasm Metastasis|
|Related Pathways||Pathogenesis; Transport; Reverse Transcription; Electron Transport; Tricarboxylic Acid Cycle; Oxidative Phosphorylation; Cell Death; Electron Transport Chain; Localization; Virulence; Fermentation; Angiogenesis; Rna Interference; Embryo Development; Cell Proliferation; Dna Repair; Glycolysis; Immune Response; Translation; Mrna Transcription|
|Background||SDHA(Succinate dehydrogenase complex, subunit a, flavoprotein), also called SDH1, HOMOLOG OF, is a protein that in humans is encoded by the SDHA gene. The SDHA gene is mapped on 5p15.33. The SDHA gene is highly polymorphic. The gene that codes for the SDHA protein is nuclear, even though the protein is located in the inner membrane of the mitochondria. Heterozygous carriers of an SDHA mutation do not developparagangliomas as has been seen for mutations in the other subunits. In vitro functional expression studies in the yeast homolog showed that the mutation resulted in a loss of SDH activity and rendered the mutant SDHA protein more susceptible to proteolysis. The findings indicated that SDHA, like other SDH subunits, can act as a tumor suppressor gene.|
|Scientific References||PMID: 7798181 by Hirawake H., et al. Human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of the flavoprotein (Fp) subunit of liver mitochondria.|
PMID: 8142412 by Morris A.A.M., et al. The cDNA sequence of the flavoprotein subunit of human heart succinate dehydrogenase.
Our Boster Quality Guarantee for Anti-SDHA Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Heart Tissue Lysate
Lane 2: COLO320 Cell Lysate
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