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Product Info Summary
| SKU: | P10102 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-Selenophosphate Synthetase 1 SEPHS1 Antibody
SKU/Catalog Number
P10102
Size
100uL
Form
Lyophilized
Description
Boster Bio Anti-Selenophosphate Synthetase 1 SEPHS1 Antibody (Catalog # P10102). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
Cite This Product
Anti-Selenophosphate Synthetase 1 SEPHS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P10102)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P10102 is reactive to SEPHS1 in Mouse
Observed Molecular Weight
52 kDa
Calculated molecular weight
42.9 kDa
Background of SEPHS1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase 1 (SPS1) (also known as selinide water dikinase or selenium donor protein 1) is the homolog of the SelD gene product in E. coli. In E. coli, SelD generates the selenium donor for selenocysteine tRNA biosynthesis. The function of SPS1 in mammals is still unknown but appears to differ from that of E. coli SelD.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P10102 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:500 - 1:2,000
This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to SPS1 by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
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Western blot using Boster's anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell (lane 1), mouse kidney, and liver tissue lysates (lanes 3-4). No signal is seen in NIH3T3 cells after pre-treatment with SPS1 siRNA (lane 2). SPS1 control (lane 5-6). Negligible cross-reactivity is seen against recombinant SPS2 (lane 7). The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Specific Publications For Anti-Selenophosphate Synthetase 1 SEPHS1 Antibody (P10102)
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1 Customer Q&As for Anti-Selenophosphate Synthetase 1 SEPHS1 Antibody
Question
We are currently using anti-Selenophosphate Synthetase 1 antibody P10102 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The anti-Selenophosphate Synthetase 1 antibody (P10102) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-20
