Product Info Summary
SKU: | A01761-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Semaphorin 3A/SEMA3A Antibody Picoband™
View all Semaphorin 3A Antibodies
SKU/Catalog Number
A01761-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Semaphorin 3A/SEMA3A Antibody Picoband™ catalog # A01761-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Semaphorin 3A/SEMA3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01761-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Semaphorin 3A recombinant protein (Position: N21-D223).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01761-1 is reactive to SEMA3A in Human, Mouse, Rat
Applications
A01761-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
88.889kDa
Background of Semaphorin 3A
SEMA3A is also known as COLL1, SEMA1 or SEMAL. This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Semaphorin 3A using anti-Semaphorin 3A antibody (A01761-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human U-87MG whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Semaphorin 3A antigen affinity purified polyclonal antibody (Catalog # A01761-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Semaphorin 3A at approximately 95KD. The expected band size for Semaphorin 3A is at 89KD.
Protein Target Info & Infographic
Gene/Protein Information For SEMA3A (Source: Uniprot.org, NCBI)
Gene Name
SEMA3A
Full Name
Semaphorin-3A
Weight
88.889kDa
Superfamily
semaphorin family
Alternative Names
(semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD SEMA3A COLL1, HH16, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SEMAL, SemD, coll-1 semaphorin 3A semaphorin-3A|collapsin 1|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A|semaphorin D|semaphorin III|semaphorin L
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEMA3A, check out the SEMA3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEMA3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Semaphorin 3A/SEMA3A Antibody Picoband™ (A01761-1)
Hello CJ!
A01761-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.
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