Product Info Summary
SKU: | A02156-4 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Sentrin-specific protease 1 SENP1 Antibody
SKU/Catalog Number
A02156-4
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Sentrin-specific protease 1 SENP1 Antibody catalog # A02156-4. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sentrin-specific protease 1 SENP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02156-4)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human SENP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02156-4 is reactive to SENP1 in Human
Applications
A02156-4 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
73.481kDa
Background of SENP1
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity.
Taihei Yamaguchi, Mol. Cell. Biol., Jun 2005; 25: 5171 - 5182.
Jinke Cheng, Mol. Cell. Biol., Jul 2004; 24: 6021 - 6028.
Molly A. Brewer, AACR Meeting Abstracts, Mar 2004; 2004: 1006.
Imke M. Veltman, Hum. Mol. Genet., Jul 2005; 14: 1955 - 1963.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of SENP1 using Anti-Sentrin-specific protease 1 SENP1 Antibody (A02156-4).
ICC staining SENP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Sentrin-specific protease 1 SENP1 Antibody A02156-4
Western blot analysis of SENP1 on K562 cell using anti-SENP1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For SENP1 (Source: Uniprot.org, NCBI)
Gene Name
SENP1
Full Name
Sentrin-specific protease 1
Weight
73.481kDa
Superfamily
peptidase C48 family
Alternative Names
EC 3.4.22; EC 3.4.22.-; SENP1; Sentrin/SUMO-specific protease SENP1; sentrin-specific protease 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2 SENP1 SuPr-2 SUMO specific peptidase 1 sentrin-specific protease 1|SUMO1/sentrin specific peptidase 1|SUMO1/sentrin specific protease 1|sentrin/SUMO-specific protease SENP1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SENP1, check out the SENP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SENP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sentrin-specific protease 1 SENP1 Antibody (A02156-4)
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No publications found for A02156-4
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