Product Info Summary
| SKU: | A00469 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-SERPINC1 Antibody (C-term)
SKU/Catalog Number
A00469
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-SERPINC1 Antibody (C-term) (Catalog # A00469). Tested in WB, IF, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SERPINC1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00469)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SERPINC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from the C-terminal region of human SERPINC1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00469 is reactive to SERPINC1 in Human, Mouse
Calculated molecular weight
52.6 kDa
Background of SERPINC1
SERPINC1 is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00469 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of SERPINC1 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of SERPINC1 Antibody (C-term) (Cat. #A00469) in mouse liver tissue lysates (35ug/lane). SERPINC1 (arrow) was detected using the purified Pab.
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Western blot analysis of lysate from human blood plasma tissue lysate, using SERPINC1 Antibody (C-term) (Cat. #A00469). A00469 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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SERPINC1 Antibody (C-term) (Cat. #A00469) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-SERPINC1 Antibody (C-term) (A00469)
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