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Product Info Summary
| SKU: | M00497 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SF2 Monoclonal Antibody
SKU/Catalog Number
M00497
BM5448 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SF2 Monoclonal Antibody catalog # M00497. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SF2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00497)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADGH-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SF2 Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF.
Reactive Species
M00497 is reactive to SRSF1 in Human, Mouse, Rat
Observed Molecular Weight
33 kDa
Calculated molecular weight
27.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00497 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: mouse spleen
Validation Images & Assay Conditions
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Western blot analysis of SF2 expression in mouse spleen lysate.
Specific Publications For Anti-SF2 Monoclonal Antibody (M00497)
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Customer Q&As
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5 Customer Q&As for Anti-SF2 Monoclonal Antibody
Question
I see that the anti-SF2 Monoclonal antibody M00497 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-28
Question
Does anti-SF2 Monoclonal antibody M00497 work for WB with brain placenta?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
According to the expression profile of brain placenta, SRSF1 is highly expressed in brain placenta. So, it is likely that anti-SF2 Monoclonal antibody M00497 will work for WB with brain placenta.
Boster Scientific Support
Answered: 2018-10-18
Question
Would anti-SF2 Monoclonal antibody M00497 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
Our lab technicians have not tested anti-SF2 Monoclonal antibody M00497 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-SF2 Monoclonal antibody M00497 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-14
Question
We are interested in to test anti-SF2 Monoclonal antibody M00497 on human brain placenta for research purposes, then I may be interested in using anti-SF2 Monoclonal antibody M00497 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
The products we sell, including anti-SF2 Monoclonal antibody M00497, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-SF2 Monoclonal antibody M00497. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-06
