Anti-SFRP1 Antibody Picoband™

sFRP-1 antibody

Boster Bio Anti-SFRP1 Antibody Picoband™ catalog # A01968-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01968-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-SFRP1 Antibody Picoband™

View all sFRP-1 Antibodies

SKU/Catalog Number

A01968-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SFRP1 Antibody Picoband™ catalog # A01968-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SFRP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01968-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human SFRP1 recombinant protein (A204-K314).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01968-2 is reactive to SFRP1 in Human, Mouse, Rat

Applications

A01968-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

35.386kDa

Background of sFRP-1

Secreted frizzled-related protein 1, also known as SFRP1, is a protein which in humans is encoded by the SFRP1 gene. It is mapped to 8p11.21. This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SFRP1 (Source: Uniprot.org, NCBI)

Gene Name

SFRP1

Full Name

Secreted frizzled-related protein 1

Weight

35.386kDa

Superfamily

secreted frizzled-related protein (sFRP) family

Alternative Names

FRP1; FRP-1FrzA; FRPSARP-2; FrzA; SARP-2; SARP2sFRP-1; Secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP1; sFRP-1 SFRP1 FRP, FRP-1, FRP1, FrzA, SARP2 secreted frizzled related protein 1 secreted frizzled-related protein 1|SARP-2|frizzled-related protein|sFRP-1|secreted apoptosis-related protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SFRP1, check out the SFRP1 Infographic

SFRP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SFRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-SFRP1 Antibody Picoband™

Question

Is a blocking peptide available for product anti-SFRP1 antibody (A01968-2)?

R. Zhao

Verified customer

Asked: 2019-01-29

Answer

We do provide the blocking peptide for product anti-SFRP1 antibody (A01968-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-29

Question

I see that the anti-SFRP1 antibody A01968-2 works with ELISA, what is the protocol used to produce the result images on the product page?

R. Gonzalez

Verified customer

Asked: 2016-10-04

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-10-04

Question

We are currently using anti-SFRP1 antibody A01968-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

G. Gonzalez

Verified customer

Asked: 2015-12-01

Answer

The anti-SFRP1 antibody (A01968-2) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-12-01

Question

Does anti-SFRP1 antibody A01968-2 work for ELISA with heart?

B. Krishna

Verified customer

Asked: 2015-01-26

Answer

According to the expression profile of heart, SFRP1 is highly expressed in heart. So, it is likely that anti-SFRP1 antibody A01968-2 will work for ELISA with heart.

Boster Scientific Support

Answered: 2015-01-26

Question

Is there a BSA free version of anti-SFRP1 antibody A01968-2 available?

G. Anderson

Verified customer

Asked: 2013-10-07

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SFRP1 antibody A01968-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-10-07

Order DetailsPrice
A01968-2

100μg

$370
A01968-2-10ug

10μg sample (liquid)

$99
A01968-2-Biotin

100 μg Biotin conjugated

$570
A01968-2-Cy3

100 μg Cy3 conjugated

$570
A01968-2-Dylight488

100 μg Dylight488 conjugated

$570
A01968-2-Dylight550

100 μg Dylight550 conjugated

$570
A01968-2-Dylight594

100 μg Dylight594 conjugated

$570
A01968-2-FITC

100 μg FITC conjugated

$570
A01968-2-HRP

100 μg HRP conjugated

$570
A01968-2-APC

100 μg APC conjugated

$670
A01968-2-PE

100 μg PE conjugated

$670
A01968-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A01968-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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